高效液相色谱法测定黄山药中伪原薯蓣皂苷
[Determination of pseudoprodioscin in Dioscorea panthaica by HPLC].
作者信息
Jing Wenguang, Zhang Qiwei, Liu An
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2616-8.
OBJECTIVE
To develop an HPLC method for the determination of pseudoprodioscin in Dioscorea panthaica and provide scientific basis for quality control of it and its preparation.
METHOD
The analysis was carried out on a Merck Purospher STAR RP-18e column eluted with acetonitrile and water as mobile phases in gradient mode. The detection wavelength was 203 nm, and the flow rate was 1.0 mL min(-1).
RESULT
The pseudoprodioscin in D. panthaica extract was well separated. The linear range is between 0.0800-4.00 microg, r =0.9999. The average recovery and RSD are 99.9% and 2.3%, respectively.
CONCLUSION
This method for quantitation of pseudoprodioscin in D. panthaica is simple, accurate and reliable and can be used for the quality control of D. panthaica and its preparations.
目的
建立高效液相色谱法测定黄山药中伪原薯蓣皂苷的含量，为黄山药及其制剂的质量控制提供科学依据。
方法
采用默克Purospher STAR RP - 18e色谱柱，以乙腈和水为流动相进行梯度洗脱。检测波长为203 nm，流速为1.0 mL·min⁻¹。
结果
黄山药提取物中的伪原薯蓣皂苷得到良好分离。线性范围为0.0800 - 4.00 μg，r = 0.9999。平均回收率和相对标准偏差分别为99.9%和2.3%。
结论
该方法用于测定黄山药中伪原薯蓣皂苷含量简便、准确、可靠，可用于黄山药及其制剂的质量控制。
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