Department of Oral Diagnostics and Surgery, School of Dentistry at Araraquara, UNESP-Universidade Estadual Paulista, Araraquara, Brazil.
DNA Cell Biol. 2010 Apr;29(4):191-200. doi: 10.1089/dna.2009.0919.
CXCR-2 is a receptor of interleukin-8, which is involved in acute and chronic inflammatory processes. Polymorphisms in the CXCR2 gene have been associated with chronic inflammatory conditions. The aim of this study was to investigate whether the +785(C/T), +1208(T/C), and +1440(G/A) single-nucleotide polymorphisms (SNPs) in the CXCR2 gene, as well as their haplotypes, are associated with susceptibility to periodontitis in Brazilians. DNA was extracted from the buccal epithelial cells of 487 individuals (control = 215; periodontitis = 272). The SNPs were investigated using the sequence-specific primer-polymerase chain reaction method. Associations between the polymorphisms and subject phenotypes were analyzed using the chi-squared statistical test, followed by univariate and multivariate logistic regression modeling. Haplotypes were reconstructed using the expectation-maximization algorithm, and differences in haplotype distribution between the groups were analyzed to estimate genetic susceptibility for periodontitis development. Univariate and multivariate analysis revealed that age, skin color, and smoking status were associated with periodontitis. The +1440 GG genotype was shown to be protective against periodontitis in both univariate and multivariate analysis (odds ratio OR = 0.42; 95% confidence interval [CI] = 0.19, 0.96). A similar relevant result for the +1440 GG was obtained in an alternative analysis considering a subgroup containing only white nonsmokers (OR = 0.37; 95% CI = 0.15, 0.92). White nonsmokers with the CTG/TCG haplotype appeared to be genetically protected against the development of periodontitis (OR = 0.29; 95% CI = 0.09, 0.89), while those carrying the CTG/TCA haplotype were more susceptible to the development of periodontitis (OR = 2.08; 95% CI = 1.24, 3.51). In conclusion, the +1440 SNP and some haplotypes are associated with periodontitis in Brazilian individuals.
CXCR-2 是白细胞介素-8 的受体，参与急性和慢性炎症过程。CXCR2 基因的多态性与慢性炎症状态有关。本研究旨在探讨 CXCR2 基因中的 +785(C/T)、+1208(T/C)和 +1440(G/A) 单核苷酸多态性 (SNP) 及其单倍型是否与巴西人牙周炎的易感性有关。从 487 名个体（对照组 215 名；牙周炎组 272 名）的口腔上皮细胞中提取 DNA。使用序列特异性引物聚合酶链反应方法检测 SNPs。采用卡方检验分析多态性与受试者表型之间的关系，然后采用单变量和多变量逻辑回归模型进行分析。使用期望最大化算法重建单倍型，并分析组间单倍型分布的差异，以估计牙周炎发病的遗传易感性。单变量和多变量分析表明，年龄、肤色和吸烟状况与牙周炎有关。+1440 GG 基因型在单变量和多变量分析中均显示出对牙周炎的保护作用（比值比[调整] = 0.42；95%置信区间[CI] = 0.19, 0.96）。在仅包含白种非吸烟者的亚组中进行替代分析时，+1440 GG 也得到了类似的相关结果（比值比 = 0.37；95%CI = 0.15, 0.92）。携带 CTG/TCG 单倍型的白种非吸烟者似乎在遗传上对牙周炎的发展具有保护作用（比值比 = 0.29；95%CI = 0.09, 0.89），而携带 CTG/TCA 单倍型的个体更容易发生牙周炎（比值比 = 2.08；95%CI = 1.24, 3.51）。总之，+1440 SNP 及其一些单倍型与巴西个体的牙周炎有关。
