Departamento de Diagnóstico e Cirurgia, Faculdade de Odontologia de Araraquara, UNESP-UNIV Estadual Paulista, SP, Brazil.
Clin Chim Acta. 2010 Sep 6;411(17-18):1264-8. doi: 10.1016/j.cca.2010.05.014. Epub 2010 May 19.
Interleukin 8 (IL-8) is a chemokine related to the initiation and amplification of acute and chronic inflammatory processes. Polymorphisms in the IL8 gene have been associated with inflammatory diseases. We investigated whether the -845(T/C) and -738(T/A) single nucleotide polymorphisms (SNPs) in the IL8 gene, as well as the haplotypes they form together with the previously investigated -353(A/T), are associated with susceptibility to chronic periodontitis.
DNA was extracted from buccal epithelial cells of 400 Brazilian individuals (control n=182, periodontitis n=218). SNPs were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Disease associations were analyzed by the chi(2) test, Exact Fisher test and Clump program. Haplotypes were reconstructed using the expectation-maximization algorithm and differences in haplotype distribution between the groups were analyzed to estimate genetic susceptibility for chronic periodontitis development.
When analyzed individually, no SNPs showed different distributions between the control and chronic periodontitis groups. Although, nonsmokers carrying the TTA/CAT (OR=2.35, 95% CI=1.03-5.36) and TAT/CTA (OR=6.05, 95% CI=1.32-27.7) haplotypes were genetically susceptible to chronic periodontitis. The TTT/TAA haplotype was associated with protection against the development of periodontitis (for nonsmokers OR=0.22, 95% CI=0.10-0.46).
Although none of the investigated SNPs in the IL8 gene was individually associated with periodontitis, some haplotypes showed significant association with susceptibility to, or protection against, chronic periodontitis in a Brazilian population.
白细胞介素 8（IL-8）是一种与急性和慢性炎症过程的启动和放大有关的趋化因子。IL8 基因中的多态性与炎症性疾病有关。我们研究了 IL8 基因中的-845（T/C）和-738（T/A）单核苷酸多态性（SNP），以及它们与之前研究的-353（A/T）一起形成的单倍型，是否与慢性牙周炎的易感性有关。
从 400 名巴西个体（对照组 182 名，牙周炎组 218 名）的颊上皮细胞中提取 DNA。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对 SNP 进行基因分型。通过卡方检验、确切 Fisher 检验和 Clump 程序分析疾病相关性。使用期望最大化算法重建单倍型，并分析两组之间单倍型分布的差异，以估计慢性牙周炎发展的遗传易感性。
单独分析时，对照组和慢性牙周炎组之间没有 SNP 显示出不同的分布。然而，携带 TTA/CAT（OR=2.35，95%CI=1.03-5.36）和 TAT/CTA（OR=6.05，95%CI=1.32-27.7）单倍型的不吸烟者对慢性牙周炎具有遗传易感性。TTT/TAA 单倍型与牙周炎的发生保护有关（不吸烟者 OR=0.22，95%CI=0.10-0.46）。
尽管 IL8 基因中没有一个被调查的 SNP 与牙周炎单独相关，但在巴西人群中，一些单倍型与慢性牙周炎的易感性或保护有关。
