Laboratoire de Pharmacognosie, Unité de Molécules d'Intérêt Biologique (UMIB UPRES EA 3660), Faculté de Pharmacie, Université de Bourgogne, Dijon, France.
Planta Med. 2010 May;76(8):818-21. doi: 10.1055/s-0029-1240727. Epub 2010 Jan 12.
Two new triterpene saponins ( 1- 2) together with three known saponins, deglucocyclamin I ( 3), cyclamin ( 4), and mirabilin ( 5), were isolated from the tubers of Cyclamen trocopteranthum. They were elucidated as 3 beta- O-{4- O-[3-hydroxyl-3-methylglutaryl]- beta-D-xylopyranosyl-(1 --> 2)- beta-D-glucopyranosyl-(1 --> 4)-[ beta-D-glucopyranosyl-(1 --> 2)]- alpha-L-arabinopyranosyl}-16 alpha-hydroxy-13 beta,28-epoxy-oleanan-30-al ( 1) and 3 beta- O-{4- O-[3-hydroxyl-3-methylglutaryl]- beta-D-xylopyranosyl-(1 --> 2)-[ beta-D-glucopyranosyl-(1 --> 6)]- beta-D-glucopyranosyl-(1 --> 4)-[ beta-D-glucopyranosyl-(1 --> 2)]- alpha-L-arabinopyranosyl}-16 alpha-hydroxy-20,30-lactone-olean-12-ene ( 2). Their structures were characterized mainly by a combination of 1D- and 2D-NMR techniques ( (1)H- (1)H COSY, TOCSY, NOESY, HSQC, and HMBC) and mass spectroscopy. Saponins 1, 3, and 4 showed a weak cytotoxic activity when tested against HT-29 and HCT 116 tumor colon cancer cells.
从天山堇菜的块茎中分离得到了两种新的三萜皂苷（1-2）和三种已知的皂苷，即去葡萄糖基环氨醇 I（3）、环氨醇（4）和米拉比林（5）。它们被阐明为 3β-O-{4-O-[3-羟基-3-甲基戊二酰基]-β-D-木吡喃糖基-（1→2）-β-D-吡喃葡萄糖基-（1→4）-[β-D-吡喃葡萄糖基-（1→2）]-α-L-阿拉伯吡喃糖基}-16α-羟基-13β，28-环氧齐墩烷-30-醇（1）和 3β-O-{4-O-[3-羟基-3-甲基戊二酰基]-β-D-木吡喃糖基-（1→2）-[β-D-吡喃葡萄糖基-（1→6）]-β-D-吡喃葡萄糖基-（1→4）-[β-D-吡喃葡萄糖基-（1→2）]-α-L-阿拉伯吡喃糖基}-16α-羟基-20,30-内酯齐墩烷-12-烯（2）。它们的结构主要通过 1D 和 2D-NMR 技术（（1）H-（1）H COSY、TOCSY、NOESY、HSQC 和 HMBC）和质谱进行了表征。皂苷 1、3 和 4 在测试对 HT-29 和 HCT 116 肿瘤结肠癌细胞的细胞毒性时表现出较弱的活性。
