鸟分枝杆菌复合群及其他分枝杆菌的多位点酶电泳分析
Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria.
作者信息
Wasem C F, McCarthy C M, Murray L W
机构信息
Department of Biology, New Mexico State University, Las Cruces 88003-0001.
出版信息
J Clin Microbiol. 1991 Feb;29(2):264-71. doi: 10.1128/jcm.29.2.264-271.1991.
Abstract
Multilocus enzyme electrophoresis analysis was used to evaluate the Mycobacterium avium complex (MAC), M. paratuberculosis, and nine other mycobacterial species. The average number of alleles per locus was 2.8 for the 35 MAC and 2 M. paratuberculosis strains which represented 24 electrophoretic types (ETs) and two distinct groups. The M. avium group was resolved into 17 ETs and contained the M. paratuberculosis ET. The M. intracellulare group consisted of six ETs. There was complete agreement between Gen-Probe identification and group placement by multilocus enzyme electrophoresis. The mean genetic diversity per locus for the 24 MAC ETs was 0.38. This procedure subdivided some serovars and, if implemented, should prove to be a powerful epidemiologic tool for the MAC. Eleven additional ETs were formed after the data for the other mycobacterial species were pooled with those for the MAC.
摘要
多位点酶电泳分析用于评估鸟分枝杆菌复合体（MAC）、副结核分枝杆菌以及其他9种分枝杆菌。35株MAC菌株和2株副结核分枝杆菌菌株代表了24种电泳类型（ETs）和两个不同的组，每个位点的平均等位基因数为2.8。鸟分枝杆菌组被分为17个ETs，其中包含副结核分枝杆菌ET。胞内分枝杆菌组由6个ETs组成。Gen-Probe鉴定结果与多位点酶电泳的分组结果完全一致。24个MAC ETs每个位点的平均遗传多样性为0.38。该方法细分了一些血清型，若加以应用，有望成为MAC强有力的流行病学工具。将其他分枝杆菌物种的数据与MAC的数据汇总后，又形成了11个额外的ETs。
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