School of Natural Product Studies, Jadavpur University, Kolkata 700 032, India.
J Ethnopharmacol. 2010 Mar 24;128(2):490-4. doi: 10.1016/j.jep.2010.01.015. Epub 2010 Jan 14.
Nelumbo nucifera Gaertn. (Nymphaeaceae) is a well-known aquatic plant which has been used for the treatment of several disorders including skin disease, cough, inflammation, fever and many other disorders.
To explore the immunomodulatory activity of extract of rhizome (NNRE) and seed (NNSE) of the plant.
The immunomodulatory activity of NNRE and NNSE was evaluated using various in vivo models including the total and differential leukocyte count (TLC and DLC), nitroblue-tetrazolium reduction (NBT) test, neutrophil adhesion test, phagocytic response and delayed type hypersensitivity (DTH) reaction. Sheep red blood cells (SRBC, 5 x 10(9)cells/ml) were used to immunize the animals. NNRE and NNSE at the doses of 100 and 300 mg/kg were administrated.
The TLC and lymphocyte count increased significantly but the neutrophil count was decreased for NNRE and NNSE treated groups compared to the control. A dose-dependent potentiation of DTH reaction induced by SRBC was observed from the extracts. The percentage of neutrophil adhesion to the nylon fiber was increased in NNRE treated groups (63.22 and 62.91%) compared to the NNSE treated group (54.86 and 54.23%). A potential phagocytic response was seen on treatment of the extracts, and significant changes were observed in the formation of formazone crystals.
This finding suggests that the extract of rhizome and seed Nelumbo nucifera stimulate defense system by modulating several immunological parameters.
莲（莲科）是一种著名的水生植物，已被用于治疗多种疾病，包括皮肤病、咳嗽、炎症、发热和许多其他疾病。
探索植物根茎（NNRE）和种子（NNSE）提取物的免疫调节活性。
使用各种体内模型评估 NNRE 和 NNSE 的免疫调节活性，包括总白细胞计数和分类白细胞计数（TLC 和 DLC）、硝基四唑蓝还原（NBT）试验、中性粒细胞黏附试验、吞噬反应和迟发型超敏反应（DTH）反应。绵羊红细胞（SRBC，5 x 10(9)个细胞/ml）用于免疫动物。NNRE 和 NNSE 以 100 和 300 mg/kg 的剂量给药。
与对照组相比，NNRE 和 NNSE 处理组的 TLC 和淋巴细胞计数显著增加，但中性粒细胞计数减少。从提取物中观察到 SRBC 诱导的 DTH 反应呈剂量依赖性增强。NNRE 处理组的中性粒细胞黏附到尼龙纤维上的百分比（63.22%和 62.91%）高于 NNSE 处理组（54.86%和 54.23%）。提取物治疗后观察到潜在的吞噬反应，并且形成 formazone 晶体的变化显著。
这一发现表明，莲根茎和种子的提取物通过调节多种免疫参数刺激防御系统。
