Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2877-2881. doi: 10.1099/ijs.0.021048-0. Epub 2010 Jan 15.
A moderately halophilic Gram-staining-negative bacterium, designated strain Y26(T), was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26(T) was observed at 15-35 °C (optimum 25-30°C), pH 6.0-8.0 (optimum pH6.5-7.5) and with 1.5-6.0 % (w/v) NaCl (optimum 2.0-3.0 %). The predominant fatty acids were C(18 : 1)ω7c (66.2 %), C(16 : 0) (12.4 %) and C(10 : 0) 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26(T) contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26(T) formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8-94.6 % sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26(T) represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26(T) (=KACC 13705(T) =DSM 22009(T)).
一株中度嗜盐革兰氏阴性细菌，被命名为 Y26(T) 菌株，从韩国泰安海岸的潮滩中分离得到。细胞是严格需氧的、运动性的球菌，有一根鞭毛，呈现过氧化氢酶和氧化酶阳性反应。Y26(T) 菌株的生长温度为 15-35 °C（最佳温度为 25-30°C），pH 值为 6.0-8.0（最佳 pH 值为 6.5-7.5），盐度为 1.5-6.0 %（w/v）（最佳盐度为 2.0-3.0 %）。主要脂肪酸为 C(18 : 1)ω7c（66.2 %）、C(16 : 0)（12.4 %）和 C(10 : 0) 3-OH（5.0 %），基因组 DNA 的 G+C 含量为 61.0 mol%。Y26(T) 菌株含有泛醌-10（Q-10）作为主要的呼吸醌。比较 16S rRNA 基因序列分析表明，Y26(T) 菌株与α变形菌纲红杆菌目中的其他属形成了一个独特的进化分支，与 Maribius、Maritimibacter 和 Palleronia 属的成员最为密切相关，序列相似度为 93.8-94.6 %。基于化学分类学数据和分子特性，Y26(T) 菌株代表了红杆菌科的一个新属，命名为 Hwanghaeicola aestuarii gen. nov.，sp. nov.。模式菌株为 Y26(T)（=KACC 13705(T) =DSM 22009(T)）。
