Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Department of Animal Science & Technology, Sunchon National University, Sunchon 540-742, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2595-2599. doi: 10.1099/ijs.0.028332-0. Epub 2010 Dec 3.
A Gram-negative bacterium, designated AD8(T), was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8(T) was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8(T) was observed at 15-40 °C (optimum 25-30 °C), at pH 5.0-9.5 (optimum pH 6.0-7.5) and with 1.0-11.0 % (w/v) NaCl (optimum 2.0-4.0 %). The predominant fatty acids were C(18 : 1)ω7c and C(19 : 0) cyclo ω8c. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8(T) belonged to the genus Citreicella and was most closely related to Citreicella marina CK-I3-6(T) within the class Alphaproteobacteria (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8(T) represents a novel species within the genus Citreicella, for which the name Citreicella aestuarii sp. nov. is proposed. The type strain is AD8(T) ( = KACC 13699(T) = DSM 22011(T)).
一株革兰氏阴性细菌，命名为 AD8(T)，从韩国泰安海岸的潮滩中分离得到。细胞是严格需氧的、运动的、卵形或杆状，一侧有两个侧生鞭毛。AD8(T)株为过氧化氢酶和氧化酶阳性，能够氧化硫代硫酸盐并将硝酸盐还原为亚硝酸盐。AD8(T)株的生长在 15-40°C（最佳温度为 25-30°C），在 pH5.0-9.5（最佳 pH6.0-7.5）和 1.0-11.0%（w/v）NaCl（最佳浓度为 2.0-4.0%）的条件下可以观察到。主要脂肪酸为 C(18 : 1)ω7c 和 C(19 : 0) 环 ω8c。基因组 DNA 的 G+C 含量为 67.5 mol%，主要醌为 Q-10。基于 16S rRNA 基因序列的系统发育分析表明，菌株 AD8(T)属于柠檬酸菌属，与α变形菌纲的海栖柠檬酸杆菌 CK-I3-6(T)最为密切相关（16S rRNA 基因序列相似度为 96.9%）。根据表型、化学分类和系统发育分析，菌株 AD8(T)代表柠檬酸菌属中的一个新种，现命名为河口柠檬酸菌。其模式菌株为 AD8(T)（=KACC 13699(T)=DSM 22011(T)）。
