在微流控装置中生成具有任意形状的氧气梯度。
Generation of oxygen gradients with arbitrary shapes in a microfluidic device.
机构信息
Department of Physics, University of California, San Diego, 9500 Gilman Drive, MC 0374, La Jolla, CA 92093, USA.
出版信息
Lab Chip. 2010 Feb 7;10(3):388-91. doi: 10.1039/b920401f. Epub 2009 Nov 27.
Abstract
We present a system consisting of a microfluidic device made of gas-permeable polydimethylsiloxane (PDMS) with two layers of microchannels and a computer-controlled multi-channel gas mixer. Concentrations of oxygen in the liquid-filled flow channels of the device are imposed by flowing gas mixtures with desired oxygen concentrations through gas channels directly above the flow channels. Oxygen gradients with different linear, exponential, and non-monotonic shapes are generated in the same liquid-filled microchannel and reconfigured in real time. The system can be used to study directed migration of cells and the development of cell and tissue cultures under gradients of oxygen.
摘要
我们提出了一个系统，该系统由透气的聚二甲基硅氧烷（PDMS）制成，包含两层微通道和一个计算机控制的多通道气体混合器。通过流经具有所需氧气浓度的混合气体直接流过微通道上方的气体通道，在充满液体的设备流道中产生氧气浓度。在相同的充满液体的微通道中产生具有不同线性、指数和非单调形状的氧气梯度，并实时重新配置。该系统可用于研究细胞在氧气梯度下的定向迁移以及细胞和组织培养的发展。
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