Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, India.
J Mater Sci Mater Med. 2010 Apr;21(4):1183-93. doi: 10.1007/s10856-010-3987-7. Epub 2010 Jan 23.
The in vitro functionality of surface phosphorylated poly(hydroxy ethyl methacrylate-co-methyl methacrylate), poly(HEMA-co-MMA) to induce bioinspired mineralization of calcium phosphate phase is evaluated. The primary nucleation of calcium phosphate on the surface phosphorylated copolymer occurs within 3 days of immersion when immersed in 1.5x simulated body fluid and the degree of mineralization is proportional to the hydroxy ethyl methacrylate content in the copolymer. The calcium phosphate phase is identified as hydroxyapatite by X-Ray diffraction analysis. The transmission electron microscopic evaluation combined with selected area diffraction pattern and energy dispersive analysis exemplified that the primary nuclei of amorphous calcium phosphate transforms to crystalline needle like calcium rich apatite, within a period of 3 days immersion in simulated body fluid. The atomic force microscopic results corroborate the c-axis growth of the crystals within 3 days immersion in SBF.
评估了表面磷酸化聚（羟乙基甲基丙烯酸酯-甲基丙烯酸甲酯）（poly(HEMA-co-MMA)）对诱导仿生磷酸钙相矿化的体外功能。当浸入 1.5x 模拟体液中时，表面磷酸化共聚物表面上磷酸钙的初始成核在浸入后的 3 天内发生，并且矿化程度与共聚物中羟乙基甲基丙烯酸酯的含量成正比。通过 X 射线衍射分析鉴定出磷酸钙相为羟基磷灰石。透射电子显微镜评估结合选区衍射图案和能量色散分析表明，在模拟体液中浸泡 3 天的时间内，无定形磷酸钙的初始核转化为富钙的针状结晶磷灰石。原子力显微镜结果证实了在 SBF 中浸泡 3 天内晶体的 c 轴生长。
