人角质形成细胞系在 Toll 样受体激活后细胞趋化因子的表达。
Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.
出版信息
Exp Dermatol. 2010 Aug;19(8):e314-6. doi: 10.1111/j.1600-0625.2009.01026.x.
Abstract
Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll-like receptor (TLR)-ligands. Expression of IL-8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1-6 and TLR9. KERTr cells responded to the same TLR-ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL-8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin.
摘要
皮肤角质形成细胞通过分泌趋化因子在先天免疫反应中发挥重要作用。本研究旨在确定角质形成细胞系是否可用于研究先天免疫机制。用一组 Toll 样受体 (TLR)-配体处理人原代角质形成细胞和 HaCaT、CCDD1106 KERTr (KERTr) 和 HEK001 细胞系。测定 IL-8、CCL20、CXCL9 和 CXCL10 的表达。这三种细胞系均表达 TLR1-6 和 TLR9。KERTr 细胞对与原代角质形成细胞相同的 TLR-配体作出反应。总体而言，HEK001 的反应相似，但似乎对鞭毛蛋白更为敏感。这与 TLR5 的表达增加一致。HaCaT 细胞中的表达谱最为独特。此外，我们的数据证实并扩展了先前关于咪喹莫特处理后角质形成细胞 TLR7 和 TLR8 独立的 IL-8 分泌的报道。不同的细胞系代表了皮肤先天免疫分子研究的互补工具。
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