双光子成像观察脑缺血时星形胶质细胞和神经元中谷胱甘肽水平的差异变化。
Differential changes of glutathione levels in astrocytes and neurons in ischemic brains by two-photon imaging.
机构信息
Department of Neurosurgery, University of New Mexico, Albuquerque, New Mexico, USA.
出版信息
J Cereb Blood Flow Metab. 2010 Apr;30(4):734-8. doi: 10.1038/jcbfm.2010.9. Epub 2010 Jan 27.
Abstract
Using two-photon imaging techniques with monochlorobimane as a glutathione (GSH) probe, we investigated GSH levels in both core and penumbra regions of an ischemic brain after middle cerebral artery occlusion. We found that the GSH level significantly decreased in the ischemic core, but increased significantly in the penumbra. Furthermore, we observed a differential change of the GSH levels in neurons and astrocytes in the penumbra. The GSH level in neurons increased significantly whereas it decreased slightly in astrocytes in the penumbra. These findings reveal critical region and cell type-dependent changes of the GSH level in an ischemic brain.
摘要
使用双光子成像技术和单氯代二氢荧光素作为谷胱甘肽 (GSH) 探针，我们研究了大脑中动脉闭塞后缺血核心和半影区的 GSH 水平。我们发现，缺血核心的 GSH 水平显著降低，但半影区的 GSH 水平显著增加。此外，我们观察到半影区神经元和星形胶质细胞中 GSH 水平的差异变化。神经元中的 GSH 水平显著增加，而星形胶质细胞中的 GSH 水平略有下降。这些发现揭示了在缺血性脑内，GSH 水平的关键区域和细胞类型依赖性变化。
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J Cereb Blood Flow Metab. 2009 Oct;29(10):1655-64. doi: 10.1038/jcbfm.2009.84. Epub 2009 Jun 24.
2
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J Cereb Blood Flow Metab. 2009 Apr;29(4):811-9. doi: 10.1038/jcbfm.2009.8. Epub 2009 Feb 18.
3
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J Neurochem. 2009 Mar;108(5):1309-21. doi: 10.1111/j.1471-4159.2009.05877.x. Epub 2009 Jan 29.
4
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J Neurochem. 2008 Jun;105(5):1849-60. doi: 10.1111/j.1471-4159.2008.05287.x. Epub 2008 Feb 7.
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7
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