Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98105-6099, USA.
Water Sci Technol. 2010;61(2):317-22. doi: 10.2166/wst.2010.819.
Human enteric viruses are important agents of waterborne illness. They are diffusely distributed in environmental waters, necessitating concentration of tens to hundreds of litres for effective detection. This study evaluates the novel ViroCap disposable capsule filter for concentration of coliphage MS2 and poliovirus (PV1) from deionised (DI) water and artificial seawater, as well as natural ground, surface, and seawater. Retention and recoveries for the ViroCap were compared with two well-characterised filters: the 1MDS for DI water, and the OptiCap XL for artificial seawater. The mean adsorption for MS2 by the ViroCap was 88%. Recovery of MS2 was significantly greater (p< or=0.01) than alternative filters tested: 65% from DI water and 63% from artificial seawater, compared to 30% for the 1MDS and 15% for the OptiCap for the respective matrices. Recovery of PV1 from DI water (37%) was similar to that of the 1MDS (51%). PV1 recoveries from artificial seawater were significantly greater (p< or =0.01) for the ViroCap (44%) than the OptiCap (11%). Recovery of MS2 from seeded environmental samples yielded 44% from groundwater, 53% from surface water, and 51% from seawater. ViroCap disposable filter is efficient for concentrating MS2 and PV1 from diverse matrices and is robust across a range of ionic concentrations.
人类肠道病毒是重要的水源性疾病病原体。它们广泛分布于环境水中，因此需要浓缩几十到几百升水样才能进行有效检测。本研究评估了新型 ViroCap 一次性囊式过滤器浓缩去离子（DI）水和人工海水中噬菌体 MS2 和脊髓灰质炎病毒（PV1），以及天然地下水、地表水和海水的效果。ViroCap 的保留率和回收率与两种特征明确的过滤器进行了比较：1MDS 用于 DI 水，OptiCap XL 用于人工海水。ViroCap 对 MS2 的平均吸附率为 88%。MS2 的回收率明显高于其他测试的替代过滤器：从 DI 水中回收 65%，从人工海水中回收 63%，而 1MDS 分别为 30%和 OptiCap 为 15%。从 DI 水中回收的 PV1（37%）与 1MDS（51%）相似。从人工海水中回收的 PV1 则明显高于 OptiCap（44%）（11%）。从接种环境样本中回收 MS2 的效率分别为地下水 44%、地表水 53%和海水 51%。ViroCap 一次性过滤器可有效浓缩多种基质中的 MS2 和 PV1，在一系列离子浓度下均具有良好的稳定性。
