Department of Prosthetic Dentistry and Oral Function, Faculty of Dentistry, University of Oslo, P.O. Box 1109 Blindern, 0317 Oslo, Norway.
Arch Oral Biol. 2010 Mar;55(3):229-34. doi: 10.1016/j.archoralbio.2010.01.003. Epub 2010 Feb 8.
The protective effect of fluoride solutions against acidic challenge on enamel was tested in vitro using calcium- and profilometer analyses.
HF-, SnF(2)-, TiF(4)- and NaF solutions (0.1M F(-), with a pH of 2.0, 2.9, 1.5, 7.3, respectively) and H(2)O were tested on a total of 60 enamel specimens divided into 10 groups. Prior to and after F-treatment five groups were exposed for 1 min to 0.01 M citric acid, and the other five groups for 10 min to 0.1M citric acid. Enamel specimens were measured by profilometry at baseline and before and after each acidic challenge. [Ca] in the collected citric acid samples was measured by atom absorption spectroscopy (AAS). DeltaCa and Delta surface height were calculated for each specimen and the data were analysed using one-way ANOVA, Tukeys test and Pearson r correlation.
AAS showed that the acidic F-treatments significantly reduced enamel dissolution for both the 1 min and 10 min acidic challenge. Profilometry showed no significant differences between the F-solutions for the 1 min groups. Significant differences could be seen between the fluorides for the 10 min groups and there was a large correlation between the profilometric and AAS results. In conclusion, all fluoride solutions reduced enamel dissolution when specimens were exposed to citric acid.
The profilometer was not sensitive enough to measure the effects of the different fluorides against a low acidic challenge. AAS was able to show these differences and for the 10 min acidic challenge there was a good correlation between the results from the two methods.
通过钙分析和轮廓仪分析，在体外测试氟化物溶液对牙釉质抗酸性侵蚀的保护作用。
共对 60 个牙釉质标本进行测试，分为 10 组，分别用 HF、SnF2、TiF4 和 NaF 溶液（0.1M F-，pH 值分别为 2.0、2.9、1.5、7.3）和 H2O 处理。在 F 处理前和处理后，有 5 组标本用 0.01M 柠檬酸分别暴露 1 分钟，另外 5 组标本用 0.1M 柠檬酸暴露 10 分钟。在基线和每次酸性挑战前后，用轮廓仪对牙釉质标本进行测量。原子吸收光谱法（AAS）测量收集的柠檬酸样品中的[Ca]。计算每个标本的 DeltaCa 和 Delta 表面高度，并使用单因素方差分析、Tukeys 检验和 Pearson r 相关性进行数据分析。
AAS 显示，在 1 分钟和 10 分钟酸性挑战中，酸性 F 处理均显著减少牙釉质溶解。轮廓仪显示，1 分钟组的 F 溶液之间无显著差异。在 10 分钟组的氟化物之间可以看到显著差异，并且轮廓仪和 AAS 结果之间存在很大的相关性。结论：所有氟化物溶液在暴露于柠檬酸时均减少牙釉质溶解。
轮廓仪对不同氟化物抵抗低酸性挑战的效果不够敏感。AAS 能够显示这些差异，并且对于 10 分钟的酸性挑战，两种方法的结果之间存在良好的相关性。
