State Key Laboratory of Marine Environmental Science, College of Ocean and EarthSciences, Xiamen University, Xiamen 361102, Fujian, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, PR China.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5818-5823. doi: 10.1099/ijsem.0.004483.
A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1, was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain O1 showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl. The predominant menaquinone was MK-8 (H), and anteiso-C and anteiso-C were the major fatty acids. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid. The DNA G+C content of strain O1 was 64.9 mol% and the genome size was 4.17 Mb. Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1 belonged to the genus . The values of average nucleotide identity and DNA-DNA hybridization between strain O1 and its close relatives were well below the thresholds used for the delineation of a new species. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is O1 (=JCM 33845=MCCC 1A16744).
一株新的革兰氏阳性、需氧、不运动、杆状放线菌，命名为 O1，从西太平洋深海沉积物中分离得到。O1 菌株在 30°C、pH 值 6.0-8.0 和 1-5%（w/v）NaCl 存在下生长最佳。主要的menaquinone 是 MK-8（H），主要的脂肪酸是 anteiso-C 和 anteiso-C。细胞壁肽聚糖中的诊断二氨基氨基酸是 -diaminopimelic 酸。主要的极性脂是双磷脂酰甘油、磷脂酰甘油和一种未知的糖脂。O1 菌株的 DNA G+C 含量为 64.9 mol%，基因组大小为 4.17 Mb。基于 16S rRNA 基因序列的相似性搜索和系统发育分析，O1 菌株属于 属。O1 菌株与其近亲之间的平均核苷酸同一性和 DNA-DNA 杂交值远低于用于划定新种的阈值。基于形态学、化学分类学特征以及基因型数据，建议 O1 菌株代表 属的一个新种，命名为. 模式菌株为 O1（=JCM 33845=MCCC 1A16744）。
