E.H. Graham Centre, Charles Sturt University and Industry & Investment NSW, PO Box 588, Wagga Wagga, NSW 2650, Australia.
Mycologia. 2010 Jan-Feb;102(1):54-61. doi: 10.3852/08-198.
A large number of isolates of Phomopsis sp. have been collected from the weed Carthamus lanatus (saffron thistle) in Australia, and their potential as biological control agents for weeds of the Asteraceae has been demonstrated. An analysis of their genetic diversity and a multigene phylogenetic analysis were undertaken to ascertain whether these isolates were distinct from other species of Phomopsis that commonly attack crop species in Australia. Minimal variation was found between the Phomopsis spp. isolated from saffron thistle, except two isolates that appeared to share identity with Diaporthe helianthii and P. viticola. Analysis of the selected isolates from saffron thistle with the nucleotide sequence of the partial ITS and tefl-alpha regions demonstrated that the sequences were distinct from all other species of Phomopsis so far described from crops in Australia. These findings provide strong support for the recognition of these isolates as a separate species of Phomopsis. The implications of these findings are discussed in relation to biological control of saffron thistle.
从澳大利亚杂草硫华菊（藏红花蓟）中收集到大量的长喙壳菌属（Phomopsis sp.）分离物，并证明它们具有作为菊科杂草生物防治剂的潜力。对其遗传多样性进行了分析，并进行了多基因系统发育分析，以确定这些分离物是否与在澳大利亚通常攻击作物的其他长喙壳菌属物种不同。从硫华菊中分离出的长喙壳菌属之间几乎没有发现变异，除了两个分离物似乎与向日葵球腔菌（Diaporthe helianthii）和葡萄座腔菌（P. viticola）具有相同的身份。对来自硫华菊的选定分离物与部分 ITS 和 tefl-α 区域的核苷酸序列进行分析表明，这些序列与迄今为止从澳大利亚作物中描述的所有其他长喙壳菌属物种都不同。这些发现为将这些分离物确认为单独的长喙壳菌属物种提供了有力支持。这些发现与硫华菊的生物防治有关。
