Domingos Marco, Dinucci Dinuccio, Cometa Stefania, Alderighi Michele, Bártolo Paulo Jorge, Chiellini Federica
Department of Chemistry & Industrial Chemistry, University of Pisa, 56126 Pisa, Italy.
Int J Biomater. 2009;2009:239643. doi: 10.1155/2009/239643. Epub 2009 Sep 8.
The most promising approach in Tissue Engineering involves the seeding of porous, biocompatible/biodegradable scaffolds, with donor cells to promote tissue regeneration. Additive biomanufacturing processes are increasingly recognized as ideal techniques to produce 3D structures with optimal pore size and spatial distribution, providing an adequate mechanical support for tissue regeneration while shaping in-growing tissues. This paper presents a novel extrusion-based system to produce 3D scaffolds with controlled internal/external geometry for TE applications.The BioExtruder is a low-cost system that uses a proper fabrication code based on the ISO programming language enabling the fabrication of multimaterial scaffolds. Poly(epsilon-caprolactone) was the material chosen to produce porous scaffolds, made by layers of directionally aligned microfilaments. Chemical, morphological, and in vitro biological evaluation performed on the polymeric constructs revealed a high potential of the BioExtruder to produce 3D scaffolds with regular and reproducible macropore architecture, without inducing relevant chemical and biocompatibility alterations of the material.
组织工程中最具前景的方法是将供体细胞接种到多孔、生物相容/可生物降解的支架上，以促进组织再生。增材生物制造工艺越来越被认为是生产具有最佳孔径和空间分布的三维结构的理想技术，在为组织再生提供足够机械支撑的同时，塑造向内生长的组织。本文提出了一种基于挤出的新型系统，用于生产具有可控内部/外部几何形状的三维支架，以用于组织工程应用。生物挤出机是一种低成本系统，它使用基于ISO编程语言的适当制造代码，能够制造多材料支架。聚（ε-己内酯）是用于生产多孔支架的材料，该支架由定向排列的微丝层制成。对聚合物构建体进行的化学、形态学和体外生物学评估表明，生物挤出机具有很高的潜力来生产具有规则且可重复的大孔结构的三维支架，而不会引起材料相关的化学和生物相容性改变。
