Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Priority Research Institute and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
J Microbiol. 2009 Dec;47(6):663-72. doi: 10.1007/s12275-009-0124-8. Epub 2010 Feb 4.
Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2 approximately 10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.
乳酸菌（LAB）是一种在人类和动物健康改善方面应用广泛的益生菌。尽管有许多益处，但由于定植和与正常菌群竞争的困难，非宿主特异性 LAB 表现出较差的益生菌效果。因此，评估了猪源 LAB 分离株作为益生菌的可行性。在 49 株乳杆菌属分离株中，有 10 株携带约 2 至 10 kb 的质粒 DNA。根据安全性测试，如溶血活性、氨、吲哚和苯丙氨酸的产生，选择了 7 株菌株。经过安全性测试后，又通过上皮细胞黏附、抗菌活性、耐酸、胆汁、热和冷干燥以及酸和过氧化氢的产生等几种测试，再次选择了 5 株菌株。然后，对酶谱（ZYM 试验）和抗生素耐药性进行分析，以进一步进行表征。通过安全性和功能测试，从猪粪便中选择了 5 株乳杆菌属的雷氏乳杆菌分离株。在这些分离株中检测到能够开发载体系统的质粒 DNA。结合这些方法，从猪中分离出了猪特异性的乳杆菌属。这些菌株可能是开发口服递送系统的有用工具。
