Assefa Senait, Ahles Kathleen, Bigelow Simone, Curtis J Thomas, Köhler Gerwald A
Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, 1111 West 17th Street, Tulsa, OK 74107 USA.
Department of Pharmacology and Physiology, Oklahoma State University Center for Health Sciences, Tulsa, USA.
Gut Pathog. 2015 Dec 30;7:35. doi: 10.1186/s13099-015-0082-0. eCollection 2015.
Recent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.
30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.
This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.
近期研究表明肠道微生物群参与肠-脑通讯，这可能带来治疗神经疾病的新方法。高度社会化的草原田鼠是研究环境因素对社会行为影响的优秀模型系统。为了未来关于益生菌在改善有社交退缩症状的疾病中作用的研究，我们报告了从田鼠中分离出的具有益生菌潜力的肠道乳酸杆菌的特性。
从田鼠肠道中分离出30株细菌菌株，通过16S rRNA基因测序和随机扩增多态性DNA指纹图谱分析发现它们与约氏乳酸杆菌不同但密切相关。进行了包括耐酸和耐胆汁、抗菌作用、抗生素敏感性以及对肠上皮细胞的黏附等体外特性分析，以评估所选菌株的益生菌潜力。由于先前研究表明摄入汞会引发田鼠的社会缺陷，因此评估了益生菌候选菌株的耐汞性，这可能是预防/治疗这些行为变化的一个重要因素。
本研究表明田鼠肠道中存在具有益生菌潜力的乳酸杆菌。本研究中鉴定出的乳酸杆菌分离株将为在田鼠行为模型系统中研究益生菌作用提供基础。
Zotero 插件
