茶树叶提取物合成的银纳米粒子（AgNps）对致病性哈维弧菌的抗菌活性及其对幼年中国对虾的保护效果。
Antibacterial activity of silver nanoparticles (AgNps) synthesized by tea leaf extracts against pathogenic Vibrio harveyi and its protective efficacy on juvenile Feneropenaeus indicus.
机构信息
Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, India.
出版信息
Lett Appl Microbiol. 2010 Apr;50(4):352-6. doi: 10.1111/j.1472-765X.2010.02799.x. Epub 2010 Jan 11.
AIMS
To determine the antibacterial potential of silver nanoparticles (AgNps) synthesized by tea leaf extract against Vibrio harveyi and its protective effect on juvenile Feneropenaeus indicus.
METHODS AND RESULTS
AgNps were synthesized by a simple procedure using tea leaf extract as the reducing agent. Bacteriological tests were performed in Luria-Bertani medium on solid agar plates and in liquid systems supplemented with V. harveyi against different concentrations of AgNps. AgNps synthesized in the present study were shown to be effective against V. harveyi isolated from F. indicus. The combined results of long- and short-term treatment of AgNps synthesized by tea leaf extract showed a 71% reduction in accumulated mortality.
CONCLUSIONS
The long-term administration of AgNps synthesized by tea leaf extracts at the concentration of 10 microg significantly reduced the mortalities in F. indicus from V. harveyi infections.
SIGNIFICANCE AND IMPACT OF THE STUDY
The AgNps synthesized by tea leaf extract may be an alternative to antibiotics in controlling V. harveyi infections.
目的
确定由茶叶提取物合成的银纳米粒子（AgNps）对哈维弧菌的抗菌潜力及其对幼年中国明对虾的保护作用。
方法和结果
采用简单的方法，使用茶叶提取物作为还原剂合成 AgNps。在含有不同浓度 AgNps 的 Luria-Bertani 培养基固体琼脂平板和液体系统中进行细菌学试验，以检测对哈维弧菌的抑制效果。本研究中合成的 AgNps 对从中国明对虾中分离出的哈维弧菌有效。茶叶提取物合成的 AgNps 进行长期和短期处理的综合结果表明，累积死亡率降低了 71%。
结论
在浓度为 10 微克时，长期给予茶叶提取物合成的 AgNps 可显著降低中国明对虾因哈维弧菌感染而导致的死亡率。
研究的意义和影响
由茶叶提取物合成的 AgNps 可能是控制哈维弧菌感染的抗生素替代品。
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