Schou C, Fernandez-Caldas E, Lockey R F, Løwenstein H
ALK Research, Hørsholm, Denmark.
J Allergy Clin Immunol. 1991 Apr;87(4):828-34. doi: 10.1016/0091-6749(91)90129-c.
A sandwich ELISA was developed to measure the concentration of cockroach allergen in the environment. The assay was based on a monospecific rabbit antibody preparation reactive with determinants shared by the important allergens, Per a I and Bla g I, from American and German cockroaches. The sensitivity was 0.2 ng Lowry protein of Per a I equivalents per milliliter, corresponding to 1 ng of Per a I equivalents per gram of dust (Per a I eq/gm). The assay did not react with noncockroach-allergen sources. Dust samples from 73 households in a cockroach-infested area were assayed. The concentration in these samples varied from below detection to 200,000 ng of Per a eq/gm of dust. Three commercial cockroach-allergen extracts all contained the allergen. The assay will be valuable for studies of the clinically relevant cockroach-allergen exposure levels and for assessment of efficacy of allergen-avoidance measures. Furthermore, the assay could be used for sanitary documentation in bakeries, restaurants, etc.
开发了一种夹心酶联免疫吸附测定法（ELISA）来测量环境中蟑螂过敏原的浓度。该测定法基于一种单特异性兔抗体制剂，它能与来自美洲蟑螂和德国蟑螂的重要过敏原Per a I和Bla g I共有的决定簇发生反应。灵敏度为每毫升0.2纳克Lowry蛋白的Per a I当量，相当于每克灰尘中1纳克的Per a I当量（Per a I eq/gm）。该测定法不与非蟑螂过敏原来源发生反应。对蟑螂滋生地区73户家庭的灰尘样本进行了检测。这些样本中的浓度从低于检测限到每克灰尘200,000纳克的Per a当量不等。三种市售蟑螂过敏原提取物均含有该过敏原。该测定法对于研究临床上相关的蟑螂过敏原暴露水平以及评估过敏原回避措施的效果将具有重要价值。此外，该测定法可用于面包店、餐馆等场所的卫生记录。
