C.U.R.I.A.MO. (Centro Universitario Ricerca Interdipartimentale Attività Motoria/University Research Center on Physical Activity), University of Perugia, Perugia, Italy.
J Endocrinol Invest. 2010 Jul-Aug;33(7):489-95. doi: 10.1007/BF03346630. Epub 2010 Feb 5.
This study was performed to establish whether only 2 sessions per week of combined aerobic and resistance exercise are enough to reduce glycated hemoglobin (HbA(1c)) and to induce changes in skeletal muscle gene expression in Type 2 diabetes mellitus (DM2) subjects with metabolic syndrome. Eight DM2 subjects underwent a 1-yr exercise program consisting of 2 weekly sessions of 140 min that combined aerobic [at 55-70% of maximal oxygen uptake (VO(2max))] and resistance circuit training [at 60-80% of 1 repetition maximum (RM)]. The training significantly improved VO(2max) (from 33.5+/-3.8 ml/kg/min to 38.2+/-3.5 ml/kg/min, p=0.0085) and muscle strength (p<0.05). Changes over baseline were significant for HbA(1c), reduced by 0.45% (p=0.0084), fasting blood glucose (from 8.8+/-1.5 to 6.9+/-2.2 mmol/l, p=0.0132), waist circumference (from 98.9+/-4.8 to 95.9+/-4.6 cm, p=0.0054), body weight (from 87.5+/-10.7 to 85.7+/-10.1 kg, p=0.0375), systolic blood pressure (from 137+/-15 to 126+/-8 mmHg, p=0.0455), total cholesterol (from 220+/-24 to 184+/-13 mg/dl, p=0.0057), and LDL-cholesterol (from 150+/-16 to 105+/-15 mg/dl, p=0.0004). Mitochondrial DNA/nuclear DNA ratio at 6 and 12 months did not change. There was a significant increase of mRNA of peroxisome proliferator- activated receptor (PPAR)-gamma after 6 months of train - ing (p=0.024); PPARalpha mRNA levels were significantly increased at 6 (p=0.035) and 12 months (p=0.044). The mRNA quantification of other genes measured [mitochondrially encoded cytochrome c oxidase subunit II (MTCO2), cytochrome c oxidase subunit Vb (COX5b), PPARgamma coactivator 1alpha (PGC- 1alpha), glucose transporter 4 (GLUT 4), forkhead transcription factor BOX O1 (FOXO-1), carnitine palmitoyltransferase 1 (CPT-1), lipoprotein lipase (LPL), and insulin receptor substrate 1 (IRS-1)] did not show significant changes at 6 and 12 months. This study suggests that a twice-per-week frequency of exercise is sufficient to improve glucose control and the expression of skeletal muscle PPARgamma and PPARalpha in DM2 subjects with metabolic syndrome.
本研究旨在确定每周仅进行 2 次有氧和抗阻运动的组合是否足以降低糖化血红蛋白（HbA1c），并诱导 2 型糖尿病（DM2）合并代谢综合征患者骨骼肌基因表达的变化。8 例 DM2 患者接受了为期 1 年的运动方案，包括每周 2 次、每次 140 分钟的运动，运动方式为有氧（最大摄氧量的 55-70%）和抗阻循环训练（1 次重复最大值的 60-80%）。训练显著提高了最大摄氧量（从 33.5+/-3.8 ml/kg/min 提高到 38.2+/-3.5 ml/kg/min，p=0.0085）和肌肉力量（p<0.05）。与基线相比，HbA1c 显著降低了 0.45%（p=0.0084），空腹血糖从 8.8+/-1.5 降至 6.9+/-2.2 mmol/l（p=0.0132），腰围从 98.9+/-4.8 降至 95.9+/-4.6 cm（p=0.0054），体重从 87.5+/-10.7 降至 85.7+/-10.1 kg（p=0.0375），收缩压从 137+/-15 降至 126+/-8 mmHg（p=0.0455），总胆固醇从 220+/-24 降至 184+/-13 mg/dl（p=0.0057），LDL-胆固醇从 150+/-16 降至 105+/-15 mg/dl（p=0.0004）。6 个月和 12 个月时线粒体 DNA/核 DNA 比值没有变化。经过 6 个月的训练，过氧化物酶体增殖物激活受体（PPAR）-γ的 mRNA 显著增加（p=0.024）；PPARalpha mRNA 水平在 6 个月（p=0.035）和 12 个月（p=0.044）时均显著增加。6 个月和 12 个月时测量的其他基因（线粒体编码细胞色素 c 氧化酶亚基 II（MTCO2）、细胞色素 c 氧化酶亚基 Vb（COX5b）、PPARγ共激活因子 1α（PGC-1α）、葡萄糖转运蛋白 4（GLUT4）、叉头转录因子 BOX O1（FOXO-1）、肉碱棕榈酰转移酶 1（CPT-1）、脂蛋白脂肪酶（LPL）和胰岛素受体底物 1（IRS-1））的 mRNA 定量没有显著变化。本研究表明，每周进行 2 次运动的频率足以改善 2 型糖尿病合并代谢综合征患者的血糖控制和骨骼肌中 PPARγ和 PPARalpha 的表达。
