Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
Toxicol In Vitro. 2010 Jun;24(4):1203-7. doi: 10.1016/j.tiv.2010.02.009. Epub 2010 Feb 10.
To approach the real haemolytic process of jellyfish toxins, both in vitro and in vivo haemolysis of tentacle-only extract (TOE) from jellyfish Cyanea capillata has been studied. Dose-response curves of the haemolytic activity of TOE in vitro were sigmoid shaped in both erythrocyte suspension and diluted whole blood, with the former more sensitive to TOE. The in vivo haemolysis increased sharply in the first 10 min and was followed by a gradual increase in the following 3h, with increasing blood potassium and lactic acid accordingly. SC5b-9 complexes were significantly up-regulated in vitro, but not in vivo. These results showed that the haemolysis of TOE in diluted whole blood and in vivo is not totally consistent with that in the erythrocyte suspension, and blood plasma might play a protective role against haemolysis. Thus we suggested that erythrocyte suspension can be used to test the damage of toxin on erythrocyte membrane, while the diluted whole blood may be more suitable to test the haemolysis of toxins.
为了研究水母毒素的真实溶血过程，我们研究了来自海月水母的触手提取物（TOE）的体外和体内溶血。TOE 的体外溶血活性剂量反应曲线在红细胞悬液和稀释全血中均呈 S 形，前者对 TOE 更为敏感。体内溶血在最初的 10 分钟内急剧增加，随后在接下来的 3 小时内逐渐增加，同时血钾和乳酸也相应增加。SC5b-9 复合物在体外显著上调，但在体内没有上调。这些结果表明，稀释全血和体内的 TOE 溶血与红细胞悬液中的不完全一致，血浆可能对溶血有保护作用。因此，我们建议可以使用红细胞悬液来测试毒素对红细胞膜的损伤，而稀释全血可能更适合测试毒素的溶血作用。
