Department of Orthopaedic Surgery, Iwate Medical University School of Medicine, Morioka, Japan.
Med Biol Eng Comput. 2010 May;48(5):469-74. doi: 10.1007/s11517-010-0586-1. Epub 2010 Feb 12.
This study was carried out to determine the effect of age on the intervertebral disc, using a rabbit model. Anulus fibrosus and nucleus pulposus tissue from New Zealand white rabbits aged 3 years old (old rabbits) and 6 months old (young rabbits) were used. The water content, the proteoglycan, the DNA content, and the mRNA levels of aggrecan, type I collagen, and type II collagen were all measured for each sample. Water, proteoglycan, DNA, and the mRNA levels of aggrecan and type II collagen were all greater in the nucleus pulposus of the young rabbits as compared to the old. For the anulus fibrosus, the difference between young and old is less marked with only proteoglycan and DNA being greater in the young disc as compared to the old. Clearly, according to our results, it is the nucleus pulposus that suffers the brunt of the changes with age.
本研究旨在通过兔模型确定年龄对椎间盘的影响。使用新西兰白兔 3 岁（老年兔）和 6 个月（幼年兔）的纤维环和髓核组织。对每个样本的水含量、蛋白聚糖、DNA 含量以及聚集蛋白聚糖、I 型胶原和 II 型胶原的 mRNA 水平进行了测量。与老年兔相比，幼年兔的髓核水、蛋白聚糖、DNA 和聚集蛋白聚糖及 II 型胶原的 mRNA 水平均较高。对于纤维环，只有年轻的纤维环中蛋白聚糖和 DNA 含量高于老年，因此年轻和老年之间的差异不太明显。显然，根据我们的结果，是髓核首先受到年龄变化的影响。
