Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Bioresour Technol. 2010 Jun;101(12):4667-75. doi: 10.1016/j.biortech.2010.01.058. Epub 2010 Feb 11.
Enrichment and separation of genistein and apigenin from extracts of pigeon pea roots were studied using eleven macroporous resins with different physical and chemical properties. ADS-5 resin showed the maximum effectiveness among the tested resins. The solute affinity towards ADS-5 resin at different temperatures was described in terms of Langmuir and Freundlich isotherms, and the equilibrium experimental data were well-fitted to the two isotherms. In order to optimize the operating parameters for separating genistein and apigenin, dynamic adsorption and desorption tests were carried out. After one run treatment with ADS-5 resin, the contents of genistein and apigenin in the product were 9.36-fold and 11.09-fold increased with recovery yields of 89.78% and 93.41%, respectively. The process achieved easy and effective enrichment and separation of genistein and apigenin by using ADS-5 resin, and it is a promising basis for large-scale preparation of genistein and apigenin from pigeon pea or other plants extracts.
采用 11 种不同物理化学性质的大孔树脂对木豆根提取物中染料木黄酮和芹菜素进行了富集和分离研究。在测试的树脂中，ADS-5 树脂的效果最好。以 Langmuir 和 Freundlich 等温线的形式描述了不同温度下溶质对 ADS-5 树脂的亲和力，并且平衡实验数据很好地符合这两种等温线。为了优化分离染料木黄酮和芹菜素的操作参数，进行了动态吸附和解吸实验。经 ADS-5 树脂一次运行处理后，产物中染料木黄酮和芹菜素的含量分别增加了 9.36 倍和 11.09 倍，回收率分别为 89.78%和 93.41%。该工艺通过 ADS-5 树脂实现了染料木黄酮和芹菜素的有效富集和分离，为从木豆或其他植物提取物中大规模制备染料木黄酮和芹菜素提供了有前景的基础。
