BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Brazil.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):60-64. doi: 10.1099/ijs.0.021105-0. Epub 2010 Feb 12.
A Gram-negative, aerobic bacterium, designated R-40509(T), was isolated from mucus of the reef builder coral (Mussismilia hispida) located in the São Sebastião Channel, São Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na(+) for growth. Its phylogenetic position was in the genus Marinobacterium and the closest related species were Marinobacterium sediminicola, Marinobacterium maritimum and Marinobacterium stanieri; the isolate exhibited 16S rRNA gene sequence similarities of 97.5-98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus Marinobacterium were below 96 %. DNA-DNA hybridizations between strain R-40509(T) and the type strains of the phylogenetically closest species of the genus Marinobacterium revealed less than 70 % DNA-DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15-42 °C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize d-alanine, l-alanine, bromosuccinic acid, β-hydroxybutyric acid and α-ketovaleric acid, but not acetate or l-arabinose. It produced acetoin (Voges-Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C(18 : 1)ω7c (35 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 25 %) and C(16 : 0) (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name Marinobacterium coralli sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509(T) (=LMG 25435(T) =CAIM 1449(T)).
一株革兰氏阴性需氧菌，命名为 R-40509(T)，从巴西圣保罗圣塞巴斯蒂昂海峡的造礁珊瑚（Mussismilia hispida）的粘液中分离得到。该菌株氧化酶阳性，过氧化氢酶阴性，生长需要 Na(+)。其系统发育位置在海洋杆菌属中，与最接近的种是海洋杆菌 sediminicola、海洋杆菌 maritimum 和海洋杆菌 stanieri；该分离株与这些种的模式株的 16S rRNA 基因序列相似性为 97.5-98.0%。与海洋杆菌属的其他模式株的 16S rRNA 基因序列相似性低于 96%。菌株 R-40509(T)与海洋杆菌属中亲缘关系最密切的种的模式株之间的 DNA-DNA 杂交显示低于 70%的 DNA-DNA 相关性，支持该菌株的新种地位。表型特征表明，该菌株能够在 15-42°C 和含有高达 9%NaCl 的培养基中生长。该分离株可通过多种特征与表型相关的种区分开来，包括其能够利用 d-丙氨酸、l-丙氨酸、溴琥珀酸、β-羟基丁酸和α-酮戊酸，但不能利用乙酸盐或 l-阿拉伯糖。它产生乙酰基甲醇（Voges-Proskauer），但没有酯酶脂肪酶（C8）或过氧化氢酶活性。它含有 C(18 : 1)ω7c (35%)、总和特征 3（异-C(15 : 0) 2-OH 和/或 C(16 : 1)ω7c；25%）和 C(16 : 0)（22%）作为主要的细胞脂肪酸。DNA G+C 含量为 58.5 mol%。提议使用 Marinobacterium coralli sp. nov. 来容纳这个新的分离株；模式株是 R-40509(T)（=LMG 25435(T) =CAIM 1449(T)）。
