The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2338-2341. doi: 10.1099/ijs.0.027326-0. Epub 2010 Oct 29.
A Gram-negative, motile, psychrotolerant, oxidase- and catalase-positive bacterium, designated BSs20135(T), was isolated from Arctic marine sediment. Cells were straight or slightly curved rods and formed circular, convex and yellowish-brown colonies. Buds and prosthecae could be produced. The strain grew at 4-28 °C (optimum 25 °C) and with 1-5 % (w/v) NaCl (optimum 2 %) and hydrolysed aesculin and DNA, but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain BSs20135(T) belonged to the genus Glaciecola and shared 93.6-97.7 % sequence similarity with the type strains of known species of the genus Glaciecola. The major cellular fatty acids of strain BSs20135(T) were summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), C(16 : 0), C(17 : 1)ω8c and C(18 : 1)ω7c. The genomic DNA G+C content was 40.3 mol%. Based on 16S rRNA gene sequence analysis, DNA-DNA hybridization data and phenotypic and chemotaxonomic characterization, strain BSs20135(T) represents a novel species, for which the name Glaciecola arctica sp. nov. is proposed. The type strain is BSs20135(T) ( = CCTCC AB 209161(T) = KACC 14537(T)).
一株革兰氏阴性、运动、嗜冷、氧化酶和过氧化氢酶阳性的细菌，被命名为 BSs20135(T)，从北极海洋沉积物中分离得到。细胞为直或略弯的杆菌，形成圆形、凸起和黄褐色的菌落。可以产生芽和菌褶。该菌株在 4-28°C（最适 25°C）和 1-5%（w/v）NaCl（最适 2%）下生长，并水解马栗树皮素和 DNA，但不能将硝酸盐还原为亚硝酸盐。16S rRNA 基因序列的系统发育分析表明，BSs20135(T)菌株属于 Glaciecola 属，与已知种的 Glaciecola 属的模式菌株的序列相似度为 93.6-97.7%。BSs20135(T)菌株的主要细胞脂肪酸为 sum feature 3（包含 C(16 : 1)ω7c 和/或 iso-C(15 : 0) 2-OH）、C(16 : 0)、C(17 : 1)ω8c 和 C(18 : 1)ω7c。基因组 DNA G+C 含量为 40.3 mol%。基于 16S rRNA 基因序列分析、DNA-DNA 杂交数据以及表型和化学分类学特征，BSs20135(T)菌株代表一个新的种，提议命名为 Glaciecola arctica sp. nov.。其模式菌株为 BSs20135(T)（ = CCTCC AB 209161(T) = KACC 14537(T)）。
