Istituto di Chimica Biomolecolare, C.N.R., via Campi Flegrei n. 34, 80078, Pozzuoli (Naples), Italy.
Extremophiles. 2010 Mar;14(2):233-40. doi: 10.1007/s00792-010-0303-x. Epub 2010 Feb 14.
A novel thermophilic, anaerobic, rod-shaped bacterium strain, designated Buff, was isolated from buffalo-dung samples collected from a buffalo-farm located in Caserta (Campania, south of Italy). Strain Buff was Gram-positive, motile and no spore-forming. The growth temperature range was 40-65 degrees C with an optimum at 60 degrees C, while pH growth range at 60 degrees C was 5.5-8.0 with an optimum at about pH 6.5. NaCl growth concentration ranged from 0 to 2.0% with an optimum at 0.5% (w/v); no growth was observed with the presence of NaCl 3.0% (w/v). The strain produced ethanol, acetate, lactate, H(2), H(2)S and CO(2) by glucose fermentation. The DNA G + C content was 34.4 mol%. As determined by 16S rRNA sequence analysis, this organism belonged to the genus Thermoanaerobacterium. On the basis of the physiological and molecular properties, we propose for strain Buff the new species designation Thermoanaerobacterium thermostercus sp. nov. This novel organism represents the first species of the genus Thermoanaerobacterium isolated from buffalo-dung. The type strain is Buff (=DSM 22141 = ATCC BAA-1776).
一株新型嗜热、厌氧、杆状细菌菌株，命名为 Buff，从位于意大利南部坎帕尼亚的一个水牛农场的水牛粪便样本中分离得到。Buff 菌株为革兰氏阳性，可运动且不产芽孢。生长温度范围为 40-65°C，最适温度为 60°C，而在 60°C 时的 pH 生长范围为 5.5-8.0，最适 pH 约为 6.5。NaCl 生长浓度范围为 0-2.0%，最适浓度为 0.5%（w/v）；当存在 3.0%（w/v）的 NaCl 时，没有观察到生长。该菌株通过葡萄糖发酵产生乙醇、乙酸、乳酸、H(2)、H(2)S 和 CO(2)。DNA G + C 含量为 34.4 mol%。通过 16S rRNA 序列分析，该生物属于 Thermoanaerobacterium 属。根据生理和分子特性，我们建议将 Buff 菌株命名为 Thermoanaerobacterium thermostercus sp. nov。这种新型生物是从水牛粪便中分离出的第一个 Thermoanaerobacterium 属物种。模式菌株为 Buff（=DSM 22141=ATCC BAA-1776）。
