Laboratory of Bacterial Genetics, National Institute of Public Health, Srobárova 48, 100 42 Prague, Czech Republic.
Res Microbiol. 2010 Apr;161(3):234-42. doi: 10.1016/j.resmic.2010.02.002. Epub 2010 Feb 13.
To determine whether the high proportion of antimicrobial resistance among hospital isolates of Pseudomonas aeruginosa in the Czech Republic is associated with the spread of multidrug-resistant clones, we investigated 108 bloodstream isolates collected prospectively in 2007. The isolates originated from 48 hospitals in 36 cities and were serotyped, tested for susceptibility to 10 anti-Pseudomonas agents and studied by multilocus sequence typing, macrorestriction analysis and class 1 integron typing. Forty-five isolates were fully susceptible, while 14 and 49 isolates were resistant to 1-2 and 3-9 agents, respectively. A total of 42 multilocus sequence types (ST) were identified, of which ST235 (serotype O11), ST175 (O4) and ST132 (O6) included 19, 16 and 5 isolates, respectively. These three STs encompassed 40 (82%) of 49 isolates resistant to more than two agents and originated from 29 hospitals in 22 cities. Isolates of the same ST had highly similar macrorestriction patterns. Twelve ST235 isolates harbored an integron variable region with the gene cassette array of aacA7-aadA6-orfD, while 15 ST175 isolates shared a region with the aadB-aadA13 array and all ST132 isolates carried a region with aacA4. A carbapenemase-encoding gene (bla(IMP-7)) was detected in a single strain (ST357). In conclusion, the multidrug resistance of Czech P. aeruginosa bloodstream isolates in 2007 was predominantly associated with three epidemic clones, one of which belongs to international clonal complex CC235.
为了确定捷克共和国铜绿假单胞菌医院分离株中高比例的抗生素耐药性是否与多药耐药克隆的传播有关，我们对 2007 年前瞻性采集的 108 株血流感染分离株进行了研究。这些分离株来自捷克共和国 36 个城市的 48 家医院，进行了血清型分型、10 种抗假单胞菌药物敏感性检测，并进行了多位点序列分型、宏基因组限制分析和 I 类整合子分型研究。45 株分离株完全敏感，14 株和 49 株分离株分别对 1-2 种和 3-9 种药物耐药。共鉴定出 42 种多位点序列型（ST），其中 ST235（血清型 O11）、ST175（O4）和 ST132（O6）分别包含 19、16 和 5 株分离株。这三种 ST 型共包含 49 株对 2 种以上药物耐药的分离株（82%），且均来自 22 个城市的 29 家医院。相同 ST 型的分离株具有高度相似的宏基因组限制图谱。12 株 ST235 分离株携带一个整合子可变区，该区域带有 aacA7-aadA6-orfD 基因盒阵列，15 株 ST175 分离株携带 aadB-aadA13 基因盒阵列，所有 ST132 分离株携带 aacA4 基因盒阵列。在一株分离株（ST357）中检测到编码碳青霉烯酶的基因（bla(IMP-7)）。总之，2007 年捷克铜绿假单胞菌血流感染分离株的多药耐药性主要与三个流行克隆相关，其中一个克隆属于国际克隆复合体 CC235。
