Delahaut P, Dubois M, Pri-Bar I, Buchman O, Degand G, Ectors F
Laboratoire d'hormonologie, Marloie, Belgium.
Food Addit Contam. 1991 Jan-Feb;8(1):43-53. doi: 10.1080/02652039109373954.
A radioimmunoassay for clenbuterol detection in cattle has been validated and used to monitor treated cattle. The tracer used was 4-amino-3,5-dichloro-alpha(tert-butylamino-methyl) benzyl alcohol (benzyl-3H)(clenbuterol) prepared by catalytic tritiation with tritium gas of 4-amino-3,5-dibromo-alpha-(tert-butylamino)-acetophenone, followed by chlorination at positions 3 and 5 in the aromatic ring. The rabbit antiserum was raised against a diazotized clenbuterol/human serum albumin conjugate. The assay described was sensitive (7.8 pg/tube) and reproducible. The intra- and inter-assay variability, which was assessed by measuring known quantities of clenbuterol in plasma, urine and faeces, was satisfactory for RIA. When this assay was used to monitor treated cattle the concentrations of clenbuterol in plasma, urine and faeces were directly related to the administered dose. The absorption and elimination of clenbuterol in cattle was rapid. Data obtained were consistent with results obtained in other species where a rapid clearance rate was also demonstrated.
一种用于检测牛体内克伦特罗的放射免疫分析法已得到验证，并用于监测接受治疗的牛。所使用的示踪剂是4-氨基-3,5-二氯-α（叔丁基氨基甲基）苄醇（苄基-³H）（克伦特罗），它是由4-氨基-3,5-二溴-α-（叔丁基氨基）苯乙酮用氚气进行催化氚化制备而成，随后在芳环的3位和5位进行氯化反应。兔抗血清是针对重氮化克伦特罗/人血清白蛋白偶联物产生的。所描述的该分析方法灵敏（7.8皮克/管）且可重复。通过测量血浆、尿液和粪便中已知量的克伦特罗来评估的批内和批间变异性对于放射免疫分析而言是令人满意的。当使用该分析方法监测接受治疗的牛时，血浆、尿液和粪便中克伦特罗的浓度与给药剂量直接相关。克伦特罗在牛体内的吸收和消除速度很快。所获得的数据与在其他物种中也显示出快速清除率的结果一致。
