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利用白细胞介素-2信使核糖核酸原位杂交技术对类风湿性滑膜炎中T细胞亚群的表型和功能进行研究。

An investigation of T-cell subset phenotype and function in the rheumatoid synovium using in situ hybridization for IL-2 mRNA.

作者信息

Warren C J, Howell W M, Bhambhani M, Cawley M I, Smith J L

机构信息

Regional Immunology Service, Southampton General Hospital, U.K.

出版信息

Immunology. 1991 Feb;72(2):250-5.

PMID:2016122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1384492/
Abstract

Cryostat sections of synovial biopsy tissue from patients with active rheumatoid arthritis (RA), spondyloarthritis (SpA) and osteoarthritis (OA) have been investigated for interleukin-2 (IL-2) mRNA and protein production. In situ hybridization using 32P-labelled oligonucleotide probes based on IL-2 gene sequences coded by exon 1 and exon 3 of the IL-2 gene revealed IL-2 mRNA over lymphoid aggregates in RA and SpA tissue. No IL-2 mRNA was detected in OA tissue and reflected the absence of lymphoid infiltrates in these tissues. Total mRNA, as detected by a 32P oligo dT probe, was found in all tissue examined. IL-2 protein product was detected by monoclonal antibody staining in SpA but not in RA or OA tissue. This is the first report of in situ studies of lymphoid function in the microenvironment of the RA synovium. Whether these data reflect the function of normal CD3+ CD4+ CD45RO+ T-cell subsets in the diseased joint remains to be resolved.

摘要

对来自患有活动性类风湿性关节炎（RA）、脊柱关节炎（SpA）和骨关节炎（OA）患者的滑膜活检组织的低温切片进行了白细胞介素-2（IL-2）mRNA和蛋白生成的研究。使用基于IL-2基因第1外显子和第3外显子编码序列的32P标记寡核苷酸探针进行原位杂交，结果显示在RA和SpA组织的淋巴样聚集物中有IL-2 mRNA。在OA组织中未检测到IL-2 mRNA，这反映出这些组织中不存在淋巴样浸润。通过32P寡聚dT探针检测发现，在所有检测的组织中均存在总mRNA。通过单克隆抗体染色在SpA组织中检测到了IL-2蛋白产物，但在RA或OA组织中未检测到。这是关于RA滑膜微环境中淋巴样功能原位研究的首篇报道。这些数据是否反映了患病关节中正常CD3 + CD4 + CD45RO + T细胞亚群的功能仍有待解决。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e43/1384492/aa914df9849d/immunology00121-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e43/1384492/835b2abbbd09/immunology00121-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e43/1384492/aa914df9849d/immunology00121-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e43/1384492/835b2abbbd09/immunology00121-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e43/1384492/aa914df9849d/immunology00121-0096-a.jpg

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本文引用的文献

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Interleukin-2 in rheumatoid arthritis: production of and response to interleukin-2 in rheumatoid synovial fluid, synovial tissue and peripheral blood.类风湿关节炎中的白细胞介素-2：类风湿性滑液、滑膜组织和外周血中白细胞介素-2的产生及反应
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Immunohistochemical studies of interleukin-2 and gamma-interferon in rheumatoid arthritis.
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Clin Exp Immunol. 1991 Dec;86(3):393-8. doi: 10.1111/j.1365-2249.1991.tb02943.x.
类风湿关节炎中白细胞介素-2和γ-干扰素的免疫组织化学研究。
Arthritis Rheum. 1985 Feb;28(2):174-81. doi: 10.1002/art.1780280212.
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Inhibitor of interleukin-2 in rheumatoid synovial fluid.类风湿性滑液中白细胞介素-2的抑制剂。
Arthritis Rheum. 1987 Feb;30(2):121-9. doi: 10.1002/art.1780300201.
5
Detection of activated T cell products in the rheumatoid joint using cDNA probes to Interleukin-2 (IL-2) IL-2 receptor and IFN-gamma.使用针对白细胞介素-2（IL-2）、IL-2受体和干扰素-γ的cDNA探针检测类风湿关节中活化的T细胞产物。
Clin Exp Immunol. 1988 Feb;71(2):295-301.
6
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