Bankole Munir A, Shittu Lukeman A J, Ahmed Titilade A, Bankole Marian N, Shittu Remilekun K, Kpela Terkula, Ashiru Oladapo A
Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos/Lagos University Teaching Hospital, Idi-araba, Lagos, Nigeria.
Afr J Tradit Complement Altern Med. 2007 Jun 10;4(4):427-33. doi: 10.4314/ajtcam.v4i4.31237.
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both gram positive and gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria.
在过去十年中，全球范围内对从药用植物中提取的提取物和生物活性化合物的深入研究增加了一倍。然而，由于对芝麻叶在传染病治疗中的有效性缺乏了解且缺乏民间说法，我们旨在确定芝麻（Sesame radiatum）和芝麻（Sesame indicum）粗叶提取物中存在的精油和木脂素的协同抗菌活性。使用琼脂扩散法研究了两种叶子的乙醇提取物、甲醇提取物和水提取物对革兰氏阳性和革兰氏阴性微生物以及酵母的体外协同抗菌活性。甲醇提取物的气相色谱 - 质谱植物化学筛选表明，精油中的主要化合物是羧酸和酚类基团，特别是人类已知的最有效的抗氧化剂，如芝麻酚、芝麻林素和芝麻素等。甲醇提取物和乙醇提取物分别对除肺炎链球菌和金黄色葡萄球菌外的所有测试致病微生物具有广谱抗菌作用，而水提取物对金黄色葡萄球菌、肺炎链球菌和白色念珠菌表现出抑制活性。结果证实了当地食用的芝麻叶提取物具有抗菌有效性的民间说法，特别是在尼日利亚许多地区对抗细菌和常见皮肤感染方面。
