Usman H, Osuji J C
Department of Chemistry, University of Maiduguri, Maiduguri, Nigeria.
Afr J Tradit Complement Altern Med. 2007 Jun 10;4(4):476-80.
The methanolic leaf extract of Newbouldia laevis was subjected to preliminary phytochemical screening and in-vitro antimicrobial tests. The extract revealed the presence of flavonoids, tannins, terpenes, steroidal and cardiac glycosides. The antimicrobial activity of the plant extract was assayed by the agar plate disc diffusion and nutrient broth dilution techniques. Test microorganisms were Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella spp. and Candida albicans; all the organisms were laboratory isolates. The extract inhibited the growth of all the test organisms especially against Klebsiella spp. and S. aureus which had mean inhibition zone of 42.3+/-1.5 and 32.3+/-1.5 mm respectively. The results showed minimum inhibitory concentration (MIC) of 1.563 mg/ml against Escherichia coli and Klebsiella spp. and 3.125 mg/ml against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. The minimal bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus was 0.39 mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhea, dysentery and as a remedy for wound healing whose causative agents are some of the organisms used in this study.
对光叶纽苞花的甲醇叶提取物进行了初步植物化学筛选和体外抗菌测试。提取物显示含有黄酮类化合物、单宁、萜类、甾体和强心苷。通过琼脂平板纸片扩散法和营养肉汤稀释法测定了植物提取物的抗菌活性。测试微生物有铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌、伤寒沙门氏菌、克雷伯氏菌属和白色念珠菌；所有菌株均为实验室分离株。提取物抑制了所有测试微生物的生长，尤其是对克雷伯氏菌属和金黄色葡萄球菌，其平均抑菌圈分别为42.3±1.5和32.3±1.5毫米。结果显示，对大肠杆菌和克雷伯氏菌属的最低抑菌浓度（MIC）为1.563毫克/毫升，对铜绿假单胞菌、金黄色葡萄球菌和伤寒沙门氏菌的最低抑菌浓度为3.125毫克/毫升。对大肠杆菌和金黄色葡萄球菌的最低杀菌浓度（MBC）为0.39毫克/毫升。本研究证实了该植物在传统医学中用于治疗胃部不适、腹泻、痢疾以及作为伤口愈合药物的用途，而本研究中使用的一些测试微生物正是导致这些疾病的病原体。
