Barupal Tansukh, Meena Mukesh, Sharma Kanika
Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India.
Biotechnol Rep (Amst). 2019 May 25;23:e00335. doi: 10.1016/j.btre.2019.e00335. eCollection 2019 Sep.
Plants produce a high diversity of natural products with a prominent function in the protection against microbial pathogens on the basis of their toxic effect on growth and reproduction. In the present study, effect of partially purified acetone fraction of leaves on various cytomorphological parameters i.e. mycelium width, conidial size, etc. of test fungi and fraction was subjected to confirming the presence of primary and secondary metabolites by rapid qualitative phytochemical tests, chromatographic methods such as TLC, column chromatography, GC-MS, etc. which were responsible for the inhibition of growth of test pathogen conidial size of decreased up to 64.76% at 0.039 μg/ml concentration of the extract. Mycelial width of increased up to 55.91% at 0.312 μg/ml concentration of the extract. Carbohydrate, steroids, volatile oils, flavonoids, and tannins were found to be present in acetone extract of leaf. Total of 7 bands were observed in TLC fingerprinting of acetone fraction. Total of 10 fractions were collected from the column chromatography. Fractions which show the most significant antifungal activity against the test fungus was subjected to further GC-MS analysis for the separation and identification of active principle. GC-MS analyses show the presence of total 6 constituents i.e. hexacosane, octadecane, docosane, heptacosane methyl, octacosane, and tetracosane.
植物产生多种多样的天然产物，这些天然产物基于对微生物病原体生长和繁殖的毒性作用，在抵御微生物病原体方面具有显著功能。在本研究中，叶片的部分纯化丙酮馏分对测试真菌的各种细胞形态学参数（即菌丝体宽度、分生孢子大小等）的影响，并且该馏分通过快速定性植物化学测试、色谱方法（如薄层色谱、柱色谱、气相色谱 - 质谱联用等）来确认初级和次级代谢产物的存在，这些代谢产物导致测试病原体的分生孢子大小在提取物浓度为0.039μg/ml时降低了64.76%。在提取物浓度为0.312μg/ml时，菌丝体宽度增加了55.91%。在叶片的丙酮提取物中发现存在碳水化合物、类固醇、挥发油、黄酮类化合物和单宁。在叶片丙酮馏分的薄层色谱指纹图谱中总共观察到7条带。从柱色谱中总共收集了10个馏分。对显示出对测试真菌具有最显著抗真菌活性的馏分进行进一步的气相色谱 - 质谱联用分析，以分离和鉴定活性成分。气相色谱 - 质谱联用分析显示总共存在6种成分，即二十六烷、十八烷、二十二烷、甲基二十七烷、二十八烷和二十四烷。
