Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana, Zabrze, Poland.
Int J Mol Sci. 2009 Dec 24;11(1):1-13. doi: 10.3390/ijms11010001.
Chalcones exhibit chemopreventive and antitumor effects. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a naturally occurring anticancer agent that induces apoptosis in cancer cells and is not toxic to normal cells. We examined the cytotoxic and apoptotic effect of five chalcones in combination with TRAIL on prostate cancer cells. The cytotoxicity was evaluated by the MTT and LDH assays. The apoptosis was determined using flow cytometry with annexin V-FITC. Our study showed that all five tested chalcones: chalcone, licochalcone-A, isobavachalcone, xanthohumol, butein markedly augmented TRAIL-mediated apoptosis and cytotoxicity in prostate cancer cells and confirmed the significant role of chalcones in chemoprevention of prostate cancer.
查耳酮具有化学预防和抗肿瘤作用。肿瘤坏死因子相关凋亡诱导配体（TRAIL）是一种天然存在的抗癌剂，可诱导癌细胞凋亡，对正常细胞无毒。我们研究了五种查耳酮与 TRAIL 联合对前列腺癌细胞的细胞毒性和凋亡作用。细胞毒性通过 MTT 和 LDH 测定评估。用 Annexin V-FITC 流式细胞术测定凋亡。我们的研究表明，五种测试的查耳酮（查尔酮、甘草查尔酮 A、异甘草查尔酮、黄腐醇、布替醇）均显著增强 TRAIL 介导的前列腺癌细胞凋亡和细胞毒性，并证实查耳酮在预防前列腺癌中的重要作用。
