College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea.
Arch Pharm Res. 2009 Dec;32(12):1681-7. doi: 10.1007/s12272-009-2203-0. Epub 2010 Feb 17.
Cytotoxicity-guided fractionation and purification of MeOH extract from Chionanthus retusus Lindl. et Paxton resulted in the isolation of compounds. Fourteen phenolic compounds were isolated from the EtOAc soluble fraction, and their structures were determined by spectroscopic analysis. Isolated compounds were identified as phillygenin (1), scopoletin (2), pinoresinol (3), kaempferol (4), aromadendrin (5), 2-(4-hydroxyphenyl)ethanol (6), 3,3',5,5',7-pentahydroxyflavanone (7), luteolin (8), quercetin (9), apigenin (10), chrysoeriol (11), phillyrin (12), oleuropein (13), (7R,8R)-guaiacylglycerol (14). All compounds except for 12 and 13 were isolated for the first time from this plant and genus Chionanthus. Anti-proliferative effect of isolated compounds were evaluated by the sulforhodamin B assay against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT15). Compounds 4 and 8-10 were significantly active with ED50 values of 1.84-6.35 microg/mL. Also, compounds 1, 3, 5 and 7 revealed cytotoxic effects at concentrations below 30 microg/mL.
从水楠（Chionanthus retusus Lindl. et Paxton）的甲醇提取物中进行细胞毒性导向的分段和纯化，得到了化合物。从乙酸乙酯可溶部分分离出 14 种酚类化合物，并通过光谱分析确定了它们的结构。分离出的化合物被鉴定为 phillygenin（1）、东莨菪内酯（2）、松脂醇（3）、山奈酚（4）、阿魏酸（5）、2-(4-羟基苯基)乙醇（6）、3,3',5,5',7-五羟基黄烷酮（7）、木樨草素（8）、槲皮素（9）、芹菜素（10）、chrysoeriol（11）、石吊兰素（12）、橄榄苦苷（13）、(7R,8R)-愈创木基甘油（14）。除 12 和 13 外，所有化合物均首次从该植物和水楠属中分离得到。通过磺酰罗丹明 B 测定法对四种人肿瘤细胞系（A549、SK-OV-3、SK-MEL-2 和 HCT15）评估了分离化合物的抗增殖作用。化合物 4 和 8-10 的 ED50 值为 1.84-6.35 μg/mL，具有显著的活性。此外，化合物 1、3、5 和 7 在低于 30 μg/mL 的浓度下也显示出细胞毒性作用。
