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五维晶体学。

Five-dimensional crystallography.

作者信息

Schmidt Marius, Graber Tim, Henning Robert, Srajer Vukica

机构信息

Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.

出版信息

Acta Crystallogr A. 2010 Mar;66(Pt 2):198-206. doi: 10.1107/S0108767309054166. Epub 2010 Feb 18.

DOI:10.1107/S0108767309054166
PMID:20164643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2824529/
Abstract

A method for determining a comprehensive chemical kinetic mechanism in macromolecular reactions is presented. The method is based on five-dimensional crystallography, where, in addition to space and time, temperature is also taken into consideration and an analysis based on singular value decomposition is applied. First results of such a time-resolved crystallographic study are presented. Temperature-dependent time-resolved X-ray diffraction measurements were conducted on the newly upgraded BioCARS 14-ID-B beamline at the Advanced Photon Source and aimed at elucidating a comprehensive kinetic mechanism of the photoactive yellow protein photocycle. Extensive time series of crystallographic data were collected at two temperatures, 293 K and 303 K. Relaxation times of the reaction extracted from these time series exhibit measurable differences for the two temperatures, hence demonstrating that five-dimensional crystallography is feasible.

摘要

本文提出了一种确定大分子反应中综合化学动力学机制的方法。该方法基于五维晶体学，除了空间和时间外，还考虑了温度，并应用了基于奇异值分解的分析方法。文中给出了这种时间分辨晶体学研究的初步结果。在先进光子源新升级的BioCARS 14-ID-B光束线上进行了温度依赖的时间分辨X射线衍射测量，旨在阐明光活性黄色蛋白光循环的综合动力学机制。在293 K和303 K这两个温度下收集了大量晶体学数据的时间序列。从这些时间序列中提取的反应弛豫时间在这两个温度下表现出可测量的差异，从而证明了五维晶体学是可行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/df98eabd8527/a-66-00198-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/fe4cf675b85a/a-66-00198-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/ec78a4d35121/a-66-00198-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/da1b40145e2d/a-66-00198-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/6af5ce8db74e/a-66-00198-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/0678c6e5fce4/a-66-00198-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/dd8be76e7447/a-66-00198-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/df98eabd8527/a-66-00198-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/fe4cf675b85a/a-66-00198-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/ec78a4d35121/a-66-00198-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/da1b40145e2d/a-66-00198-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/6af5ce8db74e/a-66-00198-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/0678c6e5fce4/a-66-00198-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/dd8be76e7447/a-66-00198-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e9/2824529/df98eabd8527/a-66-00198-fig7.jpg

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