Kettering J D, Schmidt N J, Lennette E H
J Clin Microbiol. 1977 Dec;6(6):647-9. doi: 10.1128/jcm.6.6.647-649.1977.
High-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus were consistently produced from human diploid fibroblast cells infected at a high multiplicity of virus, harvested after 7 days of incubation, and sonically treated immediately in 0.1 M glycine buffer, pH 9.5.
高滴度、敏感且稳定的人巨细胞病毒补体结合抗原,是通过用高病毒感染复数感染人二倍体成纤维细胞,在培养7天后收获,并立即在pH 9.5的0.1M甘氨酸缓冲液中进行超声处理而持续产生的。