Waner J L, Budnick J E
Clin Exp Immunol. 1977 Oct;30(1):44-9.
A method was developed for measuring the blastogenic response of human lymphocytes to human cytomegalovirus (CMV). Viral and control antigens were prepared by extracting disrupted infected and uninfected cell cultures with an alkaline buffer. Lymphocytes from ten donors with complement-fixing (CF) antibody exhibited a blastogenic response, whereas cells from ten seronegative donors did not. A relationship between the stimulation index (SI) and the results of neutralization (NT), indirect haemagglutination (IHA) or CF tests was not observed. The maximum blastogenic response occurred after 5 to 7 days of incubation and was usually greater when the cultures were supplemented with homologous plasma instead of sera. The presence of CMV antibody in the supplementary sera did not appear to affect the reactivity of the lymphocytes.
已开发出一种用于测量人淋巴细胞对人巨细胞病毒（CMV）的增殖反应的方法。通过用碱性缓冲液提取破碎的感染和未感染细胞培养物来制备病毒抗原和对照抗原。来自十名具有补体结合（CF）抗体的供体的淋巴细胞表现出增殖反应，而来自十名血清阴性供体的细胞则没有。未观察到刺激指数（SI）与中和（NT）、间接血凝（IHA）或CF试验结果之间的关系。最大增殖反应在培养5至7天后出现，并且当培养物补充同源血浆而非血清时通常更大。补充血清中CMV抗体的存在似乎不影响淋巴细胞的反应性。
