人淋巴细胞对人巨细胞病毒的增殖反应。
Blastogenic response of human lymphocytes to human cytomegalovirus.
作者信息
Waner J L, Budnick J E
出版信息
Clin Exp Immunol. 1977 Oct;30(1):44-9.
Abstract
A method was developed for measuring the blastogenic response of human lymphocytes to human cytomegalovirus (CMV). Viral and control antigens were prepared by extracting disrupted infected and uninfected cell cultures with an alkaline buffer. Lymphocytes from ten donors with complement-fixing (CF) antibody exhibited a blastogenic response, whereas cells from ten seronegative donors did not. A relationship between the stimulation index (SI) and the results of neutralization (NT), indirect haemagglutination (IHA) or CF tests was not observed. The maximum blastogenic response occurred after 5 to 7 days of incubation and was usually greater when the cultures were supplemented with homologous plasma instead of sera. The presence of CMV antibody in the supplementary sera did not appear to affect the reactivity of the lymphocytes.
摘要
已开发出一种用于测量人淋巴细胞对人巨细胞病毒（CMV）的增殖反应的方法。通过用碱性缓冲液提取破碎的感染和未感染细胞培养物来制备病毒抗原和对照抗原。来自十名具有补体结合（CF）抗体的供体的淋巴细胞表现出增殖反应，而来自十名血清阴性供体的细胞则没有。未观察到刺激指数（SI）与中和（NT）、间接血凝（IHA）或CF试验结果之间的关系。最大增殖反应在培养5至7天后出现，并且当培养物补充同源血浆而非血清时通常更大。补充血清中CMV抗体的存在似乎不影响淋巴细胞的反应性。
相似文献
1
Blastogenic response of human lymphocytes to human cytomegalovirus.人淋巴细胞对人巨细胞病毒的增殖反应。
Clin Exp Immunol. 1977 Oct;30(1):44-9.
2
An enzyme immunoassay based micro-neutralization test for titration of antibodies to human cytomegalovirus (CMV) and its correlation with direct ELISA measuring CMV IgG antibodies.一种基于酶免疫测定的微量中和试验，用于滴定人巨细胞病毒（CMV）抗体及其与检测CMV IgG抗体的直接酶联免疫吸附测定的相关性。
Biologicals. 1996 Mar;24(1):41-9. doi: 10.1006/biol.1996.0004.
3
Cell-mediated immunity to murine cytomegalovirus.
J Infect Dis. 1978 Nov;138(5):567-604.
4
5
6
A human monoclonal antibody to cytomegalovirus (CMV).一种针对巨细胞病毒（CMV）的人源单克隆抗体。
J Immunol. 1984 Oct;133(4):2202-5.
7
8
Comparison of cytomegalovirus antibodies by means of CF, ELISA and immunoblotting techniques.采用补体结合试验（CF）、酶联免疫吸附测定（ELISA）和免疫印迹技术对巨细胞病毒抗体进行比较。
Arch Immunol Ther Exp (Warsz). 1987;35(3):313-9.
9
Recognition of cytomegalovirus clinical isolate antigens by sera from cytomegalovirus-negative blood donors.巨细胞病毒阴性献血者血清对巨细胞病毒临床分离株抗原的识别。
Transfusion. 2004 Jul;44(7):1059-66. doi: 10.1111/j.1537-2995.2004.03292.x.
10
Cytomegalovirus antigenic heterogeneity can cause false-negative results in indirect hemagglutination and complement fixation antibody assays.巨细胞病毒抗原异质性可导致间接血凝试验和补体结合抗体试验出现假阴性结果。
J Clin Microbiol. 1985 Nov;22(5):768-71. doi: 10.1128/jcm.22.5.768-771.1985.
引用本文的文献
1
Human cytomegalovirus infection and antiviral immunity in septic patients without canonical immunosuppression.无典型免疫抑制的脓毒症患者中的人巨细胞病毒感染与抗病毒免疫
Med Microbiol Immunol. 2008 Jun;197(2):75-82. doi: 10.1007/s00430-008-0087-0. Epub 2008 Mar 8.
2
Blastogenic response of human lymphocytes to early antigen(s) of human cytomegalovirus.人淋巴细胞对人巨细胞病毒早期抗原的增殖反应。
Infect Immun. 1983 Sep;41(3):1084-8. doi: 10.1128/iai.41.3.1084-1088.1983.
3
Mitogenic activity in human embryonic fibroblasts early after infection by human cytomegalovirus.人巨细胞病毒感染后早期人胚胎成纤维细胞中的促有丝分裂活性。
Infect Immun. 1982 Oct;38(1):375-9. doi: 10.1128/iai.38.1.375-379.1982.
4
Comparison of in vitro lymphocyte proliferations induced by cytomegalovirus-infected human fibroblasts and cell-free cytomegalovirus.巨细胞病毒感染的人成纤维细胞和无细胞巨细胞病毒诱导的体外淋巴细胞增殖的比较。
Infect Immun. 1980 Dec;30(3):621-7. doi: 10.1128/iai.30.3.621-627.1980.
5
Acute cytomegalovirus infection and the host immune response. I. Development and maintenance of cytomegalovirus (CMV) induced in vitro lymphocyte reactivity and its relationship to the production of CMV antibodies.急性巨细胞病毒感染与宿主免疫反应。I. 巨细胞病毒（CMV）诱导的体外淋巴细胞反应性的发展与维持及其与CMV抗体产生的关系。
Clin Exp Immunol. 1980 Feb;39(2):263-71.
6
Lymphocyte blastogenesis and interferon production in adult human leukocyte cultures stimulated with cytomegalovirus antigens.用巨细胞病毒抗原刺激成人人类白细胞培养物中的淋巴细胞增殖及干扰素产生。
Infect Immun. 1980 Oct;30(1):17-22. doi: 10.1128/iai.30.1.17-22.1980.
7
Cell-mediated immune response of human lymphocytes to influenza A/USSR (H1N1) virus infection.人淋巴细胞对甲型流感病毒/苏联株（H1N1）感染的细胞介导免疫反应。
Infect Immun. 1980 Mar;27(3):867-71. doi: 10.1128/iai.27.3.867-871.1980.
8
Specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens.人单核细胞对疱疹病毒抗原的增殖反应的特异性
Infect Immun. 1978 Jun;20(3):646-51. doi: 10.1128/iai.20.3.646-651.1978.
本文引用的文献
1
Lymphocyte activation in subacute sclerosing panencephalitis virus and cytomegalovirus infections. In vitro stimulation in response to viral-infected cell lines.亚急性硬化性全脑炎病毒和巨细胞病毒感染中的淋巴细胞活化。对病毒感染细胞系的体外刺激反应。
J Exp Med. 1973 Oct 1;138(4):839-46. doi: 10.1084/jem.138.4.839.
2
Cytomegalovirus infections during acute childhood leukemia.
J Infect Dis. 1972 Nov;126(5):469-81. doi: 10.1093/infdis/126.5.469.
3
Patterns of cytomegaloviral complement-fixing antibody activity: a longitudinal study of blood donors.巨细胞病毒补体结合抗体活性模式：一项对献血者的纵向研究。
J Infect Dis. 1973 May;127(5):538-43. doi: 10.1093/infdis/127.5.538.
4
Three-day assay for human cytomegalovirus applicable to serum neutralization tests.适用于血清中和试验的人巨细胞病毒三日检测法。
Appl Microbiol. 1973 Jan;25(1):37-9. doi: 10.1128/am.25.1.37-39.1973.
5
Cytomegalovirus infection among renal allograft recipients.肾移植受者中的巨细胞病毒感染
Acta Med Scand. 1969 Jul-Aug;186(1-2):7-19. doi: 10.1111/j.0954-6820.1969.tb01432.x.
6
Cytomegalovirus infection after renal allotransplantation.肾移植术后巨细胞病毒感染
JAMA. 1967 Sep 4;201(10):725-8.
7
Recovery of cytomegalovirus from adults with neoplastic disease.从患有肿瘤疾病的成人中分离出巨细胞病毒。
Ann Intern Med. 1966 Mar;64(3):531-41. doi: 10.7326/0003-4819-64-3-531.
8
Diseases and mechanisms of persistent DNA virus infection: latency and cellular transformation.持续性DNA病毒感染的疾病与机制：潜伏及细胞转化
J Infect Dis. 1975 Aug;132(2):209-23. doi: 10.1093/infdis/132.2.209.
Zotero 插件