用于检测麻疹抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for detection of measles antibody.
作者信息
Boteler W L, Luipersbeck P M, Fuccillo D A, O'Beirne A J
出版信息
J Clin Microbiol. 1983 May;17(5):814-8. doi: 10.1128/jcm.17.5.814-818.1983.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of measles immunoglobulin G antibody (MEASELISA). This assay was found to be comparable to the measles hemagglutination inhibition (HAI) test. Approximately 500 sera from three centers were tested by MEASELISA and the HAI test. MEASELISA demonstrated values of greater than 99% for sensitivity, specificity, and accuracy. Values were very precise, with a mean coefficient of variation of 5.4%. MEASELISA values were shown by linear regression analysis to increase as HAI titers increased. A coefficient of determination of 1.00 was obtained from test center three. MEASELISA values were found to be linearly related (r2 greater than 0.97) to MEASELISA titers, thus enabling quantitation of measles antibody from a single value. Also, data are presented that show MEASELISA to be equivalent to complement fixation for evaluating paired sera for the presence of a significant increase in antibody levels to measles virus.
摘要
开发了一种用于检测麻疹免疫球蛋白G抗体的酶联免疫吸附测定法（MEASELISA）。发现该测定法与麻疹血凝抑制（HAI）试验相当。通过MEASELISA和HAI试验对来自三个中心的约500份血清进行了检测。MEASELISA在敏感性、特异性和准确性方面的表现均超过99%。数值非常精确，平均变异系数为5.4%。线性回归分析表明，随着HAI滴度的增加，MEASELISA值也增加。从测试中心三获得的决定系数为1.00。发现MEASELISA值与MEASELISA滴度呈线性相关（r2大于0.97），因此能够从单个值对麻疹抗体进行定量。此外，还提供了数据表明MEASELISA在评估成对血清中麻疹病毒抗体水平是否有显著升高方面等同于补体结合试验。
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