Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Eur J Pediatr. 2010 Aug;169(8):965-70. doi: 10.1007/s00431-010-1157-z. Epub 2010 Feb 19.
Oxidative stress is considered to be increased in obese subjects. However, the association of oxidative stress with visceral adiposity and adiponectin level is not fully understood in children. Forty-four obese Japanese children and adolescents, 28 boys and 16 girls, with median age of 9.9 years [5.2-13.8 years], and the 28 age-matched non-obese healthy controls, 15 boys and 13 girls, were enrolled in this study. The median BMI Z scores were +2.21 [1.31-4.38] for the obese subjects and -0.72 [-2.11-1.31] for the control. Plasma concentrations of 8-epi-prostaglandin F₂α (isoprostane), a marker of oxidative stress, and adiponectin fractions were assayed using ELISA. 8-epi-PGF₂α levels were significantly higher in the obese group (37.1 [4.7-112.7], median and the range) than in the control (11.5 [4.5-27.3]). In a univariate analysis, concentrations of 8-epi-PGF₂α positively correlated with visceral adipose tissue area measured by computed tomography, waist circumference, serum triglycerides, alanine aminotransferase, insulin levels, and the homeostasis of minimal assessment of insulin resistance and inversely correlated with high-density-lipoprotein cholesterol and high-molecular weight (HMW) adiponectin. Total-, medium-, or low-molecular weight adiponectin fraction did not show a significant correlation with 8-epi-PGF₂α Forty of 44 obese children had one or more metabolic complications. The 8-epi-PGF[Formula: see text] levels also elevated with increasing numbers of obesity-related complications. These results suggest that oxidative stress is enhanced in relation to visceral fat accumulation and decreasing HMW adiponectin level in childhood obesity. Oxidative stress may be associated with the development of obesity-related complications.
氧化应激被认为在肥胖人群中增加。然而，在儿童中，氧化应激与内脏脂肪堆积和脂联素水平的关系尚未完全阐明。本研究纳入了 44 名肥胖的日本儿童和青少年，其中 28 名为男孩，16 名为女孩，中位年龄为 9.9 岁（5.2-13.8 岁），以及 28 名年龄匹配的非肥胖健康对照者，其中 15 名为男孩，13 名为女孩。肥胖组的 BMI Z 评分中位数为+2.21[1.31-4.38]，对照组为-0.72[-2.11-1.31]。使用 ELISA 法测定血浆 8-epi-前列腺素 F₂α（异前列烷），一种氧化应激标志物，和脂联素片段的浓度。肥胖组的 8-epi-PGF₂α 水平明显高于对照组（37.1[4.7-112.7]，中位数和范围）。在单变量分析中，8-epi-PGF₂α 浓度与计算机断层扫描测量的内脏脂肪组织面积、腰围、血清甘油三酯、丙氨酸氨基转移酶、胰岛素水平、胰岛素抵抗的最小评估的稳态呈正相关，与高密度脂蛋白胆固醇和高分子量（HMW）脂联素呈负相关。总脂联素、中脂联素或低分子脂联素与 8-epi-PGF₂α 无显著相关性。44 名肥胖儿童中有 40 名患有一种或多种代谢并发症。随着肥胖相关并发症数量的增加，8-epi-PGF[Formula: see text]水平也升高。这些结果表明，氧化应激与内脏脂肪堆积和 HMW 脂联素水平降低有关，在儿童肥胖中增强。氧化应激可能与肥胖相关并发症的发生有关。
