NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):412-416. doi: 10.1099/ijs.0.020263-0. Epub 2010 Mar 26.
A Gram-stain-negative, aerobic, yellow-pigmented, heterotrophic, sulfur-oxidizing, non-motile, rod-shaped bacterium, designated KMM 3898(T), was isolated from an internal tissue of the sand snail Umbonium costatum, collected from the shallow sediments of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KMM 3898(T) formed a distinct phylogenetic lineage within the class Gammaproteobacteria and was most closely related to Leucothrix mucor DSM 2157(T) (89.2 % 16S rRNA gene sequence similarity) and members of the genus Thiothrix (86.7-88.5 %). Chemotaxonomically, strain KMM 3898(T) contained the isoprenoid quinone Q-8, the polar lipids phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown phospholipid and the fatty acids C(18 : 1)ω7c, C(16 : 1)ω7c and C(16 : 0) as predominant components (>10 %). The DNA G+C content of strain KMM 3898(T) was 43.4 mol%. On the basis of phenotypic features and phylogenetic analysis, strain KMM 3898(T) represents a novel genus and species, for which the name Cocleimonas flava gen. nov., sp. nov. is proposed. The type strain is KMM 3898(T) (=NRIC 0757(T) =JCM 16494(T)).
一株革兰氏阴性、需氧、黄色素、异养、硫氧化、非运动、杆状细菌，被命名为 KMM 3898(T)，从采自日本海浅沉积物中的沙蜗牛 Umbonium costatum 的内部组织中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KMM 3898(T)在γ变形杆菌纲中形成一个独特的系统发育谱系，与 Leucothrix mucor DSM 2157(T)（89.2% 16S rRNA 基因序列相似性）和 Thiothrix 属的成员最为密切相关（86.7-88.5%）。化学分类学上，菌株 KMM 3898(T)含有异戊烯醌 Q-8、极性脂磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和一种未知的磷脂以及脂肪酸 C(18:1)ω7c、C(16:1)ω7c 和 C(16:0)作为主要成分（>10%）。菌株 KMM 3898(T)的 DNA G+C 含量为 43.4 mol%。基于表型特征和系统发育分析，菌株 KMM 3898(T)代表一个新属和新种，因此提议建立 Cocleimonas flava 属，新种。模式株为 KMM 3898(T)=NRIC 0757(T)=JCM 16494(T)。
