新型高效重组表达系统用于工程改造南极假丝酵母脂肪酶 B。
New efficient recombinant expression system to engineer Candida antarctica lipase B.
机构信息
Ingénierie des Systèmes Biologiques et des Procédés, Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France.
出版信息
Appl Environ Microbiol. 2010 Apr;76(8):2684-7. doi: 10.1128/AEM.03057-09. Epub 2010 Feb 19.
Abstract
Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.
摘要
在这里，我们报告了使用 Yarrowia lipolytica 作为一种通用表达宿主，开发蛋白质工程方法来修饰南极假丝酵母脂肪酶 B 的性质。使用饱和突变文库定义和验证了一种可靠的筛选方案，得到的突变体显示出比野生型酶更高的催化效率。
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