利用金颗粒包被的底物将图像模式化的分子递送至活细胞中。
Image patterned molecular delivery into live cells using gold particle coated substrates.
作者信息
Wu Ting-Hsiang, Kalim Sheraz, Callahan Caitlin, Teitell Michael A, Chiou Pei-Yu
机构信息
Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.
出版信息
Opt Express. 2010 Jan 18;18(2):938-46. doi: 10.1364/OE.18.000938.
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.
通过对固定在细胞单层下方基质上的金颗粒进行脉冲激光照射，展示了一种用于哺乳动物细胞的图像图案化分子递送系统。使用时间分辨成像系统捕获图案化的空化泡成核，并通过观察膜不可渗透的荧光染料钙黄绿素的摄取来验证分子递送。观察到递送效率高达90%，并证明了多重图案化染料递送。
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