Department of Physics, King's College London, Strand, London WC2R 2LS, UK.
J Am Chem Soc. 2010 Mar 24;132(11):3989-96. doi: 10.1021/ja1002179.
Semiconducting polymer nanospheres (SPNs) have been synthesized and encapsulated in phospholipid micelles by a solvent evaporation technique. Four different conjugated polymers were used, yielding aqueous dispersions of nanoparticles which emit across the visible spectrum. The synthesis was simple and easily reproducible, and the resultant nanoparticle solutions exhibited high colloidal stability. As these encapsulated SPNs do not contain any toxic materials and show favorable optical properties, they appear to be a promising imaging agent in biomedical and imaging applications. The SPNs were used in simple fluorescence imaging experiments and showed uptake in SH-SY5Y neuroblastoma and live HeLa cells. Carboxylic acid functionalized SPNs were also synthesized and conjugated to bovine serum albumin (BSA) by carbodiimide-mediated chemistry, a key step in the realization of targeted imaging using conjugated polymers.
半导体聚合物纳米球（SPNs）已通过溶剂蒸发技术被合成并包封在磷脂胶束中。使用了四种不同的共轭聚合物，得到了在整个可见光谱范围内发射光的纳米粒子的水分散体。该合成方法简单且易于重现，所得的纳米粒子溶液表现出高胶体稳定性。由于这些包封的 SPN 不包含任何有毒材料并且表现出有利的光学性质，因此它们似乎是生物医学和成像应用中很有前途的成像剂。将 SPN 用于简单的荧光成像实验中，并显示出在 SH-SY5Y 神经母细胞瘤和活 HeLa 细胞中的摄取。还通过碳二亚胺介导的化学将羧酸官能化的 SPN 合成并连接到牛血清白蛋白（BSA）上，这是使用共轭聚合物实现靶向成像的关键步骤。
