Moser V C, McDaniel K L, Phillips P M
NSI Technology Services, Inc., Research Triangle Park, North Carolina 27709.
Toxicol Appl Pharmacol. 1991 Apr;108(2):267-83. doi: 10.1016/0041-008x(91)90117-w.
A functional observational battery (FOB) was utilized to assess the effects of 3-day exposure to the formamidine pesticide amitraz in outbred Sprague-Dawley-derived and inbred Fischer-344-derived (F344) rats (both from Charles River Laboratories) and in outbred Long-Evans rats obtained from two commercial suppliers (Charles River Breeding Laboratories and Blue Spruce Farms). Significant strain and stock differences were obtained in baseline values for one-third of the FOB measures. In most cases, F344 rats were different from the others. Characteristic signs of amitraz exposure consisting of increased excitability, hyper-reactivity, and physiological and autonomic changes were evident in all treated rats. These effects increased with repeated dosing, and many were still present 6 days after dosing. On individual measures, there were differences between the strains and stocks in terms of sensitivity and time course of amitraz effects. In general, Blue Spruce Long-Evans rats displayed more effects of amitraz and F344 rats recovered more quickly than others. Although Sprague-Dawley rats showed the least effect overall, they displayed the largest increases in the sensorimotor responses to stimuli. These data indicate that although some behavioral and physiological parameters showed strain and supplier differences, in both baseline values and the effects of amitraz, conclusions concerning its neurotoxic potential in a screening context would be similar.
使用功能观测组合（FOB）评估了3天接触甲脒类杀虫剂双甲脒对远交斯普拉格-道利衍生大鼠和近交费希尔344衍生（F344）大鼠（均来自查尔斯河实验室）以及从两家商业供应商（查尔斯河繁殖实验室和蓝云杉农场）获得的远交长-伊文斯大鼠的影响。在三分之一的FOB测量指标的基线值中发现了显著的品系和种群差异。在大多数情况下，F344大鼠与其他大鼠不同。在所有接受治疗的大鼠中，均明显出现了双甲脒接触的特征性体征，包括兴奋性增加、反应过度以及生理和自主神经变化。这些影响随着重复给药而增加，并且在给药后6天许多影响仍然存在。在个体测量指标上，品系和种群在双甲脒作用的敏感性和时间进程方面存在差异。总体而言，蓝云杉长-伊文斯大鼠表现出更多的双甲脒作用，并且F344大鼠比其他大鼠恢复得更快。尽管斯普拉格-道利大鼠总体上表现出的影响最小，但它们对刺激的感觉运动反应增加幅度最大。这些数据表明，尽管一些行为和生理参数在基线值和双甲脒作用方面表现出品系和供应商差异，但在筛选背景下关于其神经毒性潜力的结论将是相似的。
