从铺地黍和匙羹藤叶中分离得到的皂苷的杀利什曼原虫活性。
Leishmanicidal activity of saponins isolated from the leaves of Eclipta prostrata and Gymnema sylvestre.
机构信息
School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu, India.
出版信息
Indian J Pharmacol. 2009 Feb;41(1):32-5. doi: 10.4103/0253-7613.48891.
OBJECTIVE
To evaluate the leishmanicidal activity of saponin, dasyscyphin C of Eclipta prostrata and sapogenin, gymnemagenol from Gymnema sylvestre leaves under in vitro conditions.
MATERIALS AND METHODS
Dasyscyphin C/Gymnemagenol were dissolved in phosphate buffered saline (PBS) and diluted with liquid medium to obtain concentrations ranging from 1000 to 15 mug /ml. The leishmanicidal activity against leishmanial parasites, Leishmania major, Leishmania aethiopica and Leishmania tropica promastigotes was studied by the MTS assay.
RESULT
The Dasyscyphin C isolated from E. prostrata showed good leishmanicidal activity at 1000mug/ml concentration, with the IC(50) value of 450mug/ml against L. major promastigote and the percentage of parasitic death was 73; whereas, gymnemagenol of G. sylvestre showed only 52% parasitic death at 1000 mug/ml concentration. The other Leishmania species, L. aethiopica and L. tropica promastigotes, were less sensitive to the saponins of E. prostrata and G. sylvestre.
CONCLUSION
From this study, it can be concluded that the dasyscyphin C of E. prostrata has significant leishmanicidal activity against L. major promastigote.
目的
评估黄花蒿中的甾体皂苷、土木香内酯 C 和匙羹藤中的甾体皂苷元、gymnemagenol 在体外条件下的杀利什曼原虫活性。
材料和方法
将土木香内酯 C/gymnemagenol 溶解在磷酸盐缓冲盐水（PBS）中，并在液体培养基中稀释，以获得浓度范围为 1000 至 15 μg/ml 的溶液。使用 MTS 测定法研究了这些化合物对利什曼原虫寄生虫（包括大沙鼠利什曼原虫、埃塞俄比亚利什曼原虫和热带利什曼原虫）前鞭毛体的杀利什曼原虫活性。
结果
从黄花蒿中分离得到的土木香内酯 C 在 1000 μg/ml 浓度下表现出良好的杀利什曼原虫活性，对大沙鼠利什曼原虫前鞭毛体的 IC50 值为 450 μg/ml，寄生虫死亡率为 73%；而匙羹藤中的 gymnemagenol 仅在 1000 μg/ml 浓度下表现出 52%的寄生虫死亡率。其他利什曼原虫物种，即埃塞俄比亚利什曼原虫和热带利什曼原虫前鞭毛体，对黄花蒿和匙羹藤的甾体皂苷的敏感性较低。
结论
从这项研究可以得出结论，黄花蒿中的土木香内酯 C 对大沙鼠利什曼原虫前鞭毛体具有显著的杀利什曼原虫活性。
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