Hansen M S, Pors S E, Jensen H E, Bille-Hansen V, Bisgaard M, Flachs E M, Nielsen O L
Department of Veterinary Disease Biology, Faculty of Life Sciences (LIFE), University of Copenhagen, Frederiksberg C, Denmark.
J Comp Pathol. 2010 Aug-Oct;143(2-3):120-31. doi: 10.1016/j.jcpa.2010.01.012. Epub 2010 Feb 23.
Respiratory infections are among the most important diseases of growing pigs. In order to elucidate the multifactorial aetiology of porcine respiratory disease complex (PRDC) in Denmark, lungs from 148 finishing pigs with cranioventral bronchopneumonia (case group) and 60 pigs without lung lesions (control group) were collected from abattoirs. The pathogens involved in PRDC and their interactions were identified and linked to the histopathological diagnosis. The lung samples were cultured for bacteria and tested by multiplex polymerase chain reaction for presence of swine influenza virus (type A), porcine reproductive and respiratory syndrome virus (both European and US type), porcine circovirus type 2 (PCV2), porcine respiratory coronavirus, porcine cytomegalovirus, Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. All cases had cranioventral lobular bronchopneumonia consistent with PRDC. There was a broad range of microscopical lesions and the cases were characterized as acute (n=10), subacute (n=24) or chronic (n=114) bronchopneumonia. Five bacterial species, five viruses and two Mycoplasma spp. were detected in different combinations. PCV2, M. hyopneumoniae, M. hyorhinis and Pasteurella multocida were detected most frequently among the PRDC affected swine and the diversity and number of pathogens were higher in these animals compared with controls. No clear-cut associations were detected between pathogens and histological lesions or histopathological diagnoses. PRDC occurs more frequently than enzootic pneumonia among Danish finishing pigs and has complex and varied histopathology.
呼吸道感染是生长猪最重要的疾病之一。为了阐明丹麦猪呼吸道疾病综合征（PRDC）的多因素病因，从屠宰场收集了148头患有颅腹侧支气管肺炎的育肥猪（病例组）和60头无肺部病变的猪（对照组）的肺脏。确定了PRDC中涉及的病原体及其相互作用，并将其与组织病理学诊断联系起来。对肺样本进行细菌培养，并通过多重聚合酶链反应检测是否存在甲型流感病毒、猪繁殖与呼吸综合征病毒（欧洲型和美国型）、猪圆环病毒2型（PCV2）、猪呼吸道冠状病毒、猪巨细胞病毒、猪肺炎支原体和猪鼻支原体。所有病例均有与PRDC一致的颅腹侧小叶支气管肺炎。有广泛的微观病变，病例分为急性（n = 10）、亚急性（n = 24）或慢性（n = 114）支气管肺炎。检测到五种细菌、五种病毒和两种支原体以不同组合存在。在受PRDC影响的猪中，PCV2、猪肺炎支原体、猪鼻支原体和多杀性巴氏杆菌检测频率最高，与对照组相比，这些动物中病原体的多样性和数量更高。在病原体与组织学病变或组织病理学诊断之间未检测到明确的关联。在丹麦育肥猪中，PRDC的发生频率高于地方流行性肺炎，并且具有复杂多样的组织病理学特征。
