National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Nanjing, China.
Planta. 2010 Mar;231(4):875-85. doi: 10.1007/s00425-009-1094-0.
Chlorophyll a fluorescence parameters can provide qualitative and quantitative information about photosynthetic processes in chloroplasts. JIP-test and modulated fluorescence (MF) parameters are commonly used chlorophyll a fluorescence parameters. This study was conducted to identify quantitative trait loci (QTLs) associated with JIP-test parameters, MF parameters, and photosynthetic rate (P(N)), and to examine the relationships among them in soybean (Glycine max (L.) Merr.). Pot and field experiments were performed to evaluate 184 recombinant inbred lines (RILs) for five JIP-test parameters (ABS/RC, TR(O)/ABS, ET(O)/TR(O), RE(O)/ET(O), and PI(ABS)), four MF parameters (Fv/Fm, Fv'/Fm', PhiPSII, and qP), and P(N).Significant correlations were commonly observed among JIP-test parameters, MF parameters, and P(N). QTL mapping analysis identified 13, 9, and 4 QTLs for JIP-test parameters, MF parameters, and P(N), respectively, of which 13 were stable. Four major genomic regions were detected: LG A2 (19.81 cM) for JIP-test parameters, LG C1 (94.31 and 97.61 cM) for P(N) and MF parameters, LG M (100.51 cM) for JIP-test and MF parameters, and LG O (30.61-49.91 cM) for P(N), JIP-test, and MF parameters. These results indicate that chlorophyll fluorescence parameters, especially PHIPSII and qP, could play an important role in regulating P(N), and that JIP-test and MF parameters could be controlled by the same or different genes. The QTLs identified in this study will help in the understanding of the genetic basis of photosynthetic processes in plants. They will also contribute to the development of marker-assisted selection breeding programs for photosynthetic capacity in soybean.
叶绿素 a 荧光参数可为叶绿体光合作用过程提供定性和定量信息。JIP 测试和调制荧光（MF）参数是常用的叶绿素 a 荧光参数。本研究旨在鉴定与 JIP 测试参数、MF 参数和光合速率（P（N））相关的数量性状位点（QTL），并研究它们之间的关系。在大豆（Glycine max（L.）Merr.）中进行了盆栽和田间试验，以评估 184 个重组自交系（RIL）的五个 JIP 测试参数（ABS/RC、TR（O）/ABS、ET（O）/TR（O）、RE（O）/ET（O）和 PI（ABS））、四个 MF 参数（Fv/Fm、Fv'/Fm'、PhiPSII 和 qP）和 P（N）。JIP 测试参数、MF 参数和 P（N）之间通常存在显著相关性。QTL 作图分析鉴定了 13、9 和 4 个 JIP 测试参数、MF 参数和 P（N）的 QTL，其中 13 个是稳定的。检测到四个主要基因组区域：LG A2（19.81 cM）用于 JIP 测试参数，LG C1（94.31 和 97.61 cM）用于 P（N）和 MF 参数，LG M（100.51 cM）用于 JIP 测试和 MF 参数，以及 LG O（30.61-49.91 cM）用于 P（N）、JIP 测试和 MF 参数。这些结果表明，叶绿素荧光参数，特别是 PhiPSII 和 qP，可能在调节 P（N）中发挥重要作用，并且 JIP 测试和 MF 参数可能由相同或不同的基因控制。本研究中鉴定的 QTL 将有助于理解植物光合作用过程的遗传基础。它们还将为大豆光合能力的标记辅助选择育种计划做出贡献。
