Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Chem Biol Interact. 2010 Apr 15;185(1):59-65. doi: 10.1016/j.cbi.2010.02.028. Epub 2010 Feb 24.
Excess visceral adiposity may predispose to chronic diseases like hypertension and type 2 diabetes with a high risk for coronary artery disease. Adipose tissue secreted cytokines and oxidative stress play an important role in chronic disease progression. To combat adiposity, plant-derived triterpenes are currently receiving much attention as they possess antioxidant and anti-inflammatory properties and the ability to regulate glucose and lipid metabolism. In the search for potential antiobese compounds from natural sources, this study evaluated the effects of oleanolic acid (OA), a pentacyclic triterpene commonly present in fruits and vegetables, in glucose tolerance test and on high-fat diet (HFD)-induced obesity in mice. Adult male Swiss mice treated or not with OA (10 mg/kg) were fed a HFD during 15 weeks. Sibutramine (SIB) treated group (10 mg/kg) was included for comparison. Weekly body weights, food and water consumption were measured, and at the end of study period, the levels of blood glucose and lipids, plasma hormone levels of insulin, ghrelin and leptin, and the visceral abdominal fat content were analysed. Mice treated with OA and fed a HFD showed significantly (p<0.05) improved glucose tolerance, decreased body weights, visceral adiposity, blood glucose, plasma lipids relative to their respective controls fed no OA. Additionally, OA treatment, while significantly elevating the plasma hormone level of leptin, decreased the level of ghrelin. However, it caused a greater decrease in plasma amylase activity than lipase. Sibutramine-treated group also manifested similar effects like OA except for blood glucose level that was not different from HFD control. These findings suggest that OA ameliorates visceral adiposity and improves glucose tolerance in mice and thus has an antiobese potential through modulation of carbohydrate and fat metabolism.
内脏脂肪过多可能导致高血压和 2 型糖尿病等慢性疾病，并增加患冠状动脉疾病的风险。脂肪组织分泌的细胞因子和氧化应激在慢性疾病进展中发挥重要作用。为了对抗肥胖，植物源性三萜类化合物目前受到广泛关注，因为它们具有抗氧化和抗炎特性，并且能够调节葡萄糖和脂质代谢。在从天然来源寻找潜在的抗肥胖化合物的研究中，本研究评估了齐墩果酸（OA）对葡萄糖耐量试验和高脂肪饮食（HFD）诱导的肥胖的影响，OA 是一种五环三萜，常见于水果和蔬菜中。成年雄性瑞士小鼠用或不用 OA（10mg/kg）处理，并用 HFD 喂养 15 周。纳入西布曲明（SIB）处理组（10mg/kg）进行比较。每周测量体重、食物和水的消耗，在研究期末，分析血糖和血脂水平、血浆胰岛素、胃饥饿素和瘦素激素水平以及内脏腹部脂肪含量。用 HFD 喂养并用 OA 处理的小鼠表现出明显（p<0.05）改善的葡萄糖耐量、体重减轻、内脏肥胖、血糖、血浆脂质，与未用 OA 喂养的对照组相比。此外，OA 治疗虽然显著升高了血浆瘦素激素水平，但降低了胃饥饿素水平。然而，它导致血浆淀粉酶活性的下降大于脂肪酶。西布曲明处理组也表现出与 OA 相似的作用，除了血糖水平与 HFD 对照组没有差异。这些发现表明，OA 通过调节碳水化合物和脂肪代谢，改善了小鼠的内脏肥胖和葡萄糖耐量，因此具有抗肥胖的潜力。
